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DC Field | Value | Language |
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dc.contributor.author | García-Risco, Mario | - |
dc.contributor.author | Calatayud, Sara | - |
dc.contributor.author | Pedrini-Martha, Veronika | - |
dc.contributor.author | Albalat Rodríguez, Ricard | - |
dc.contributor.author | Dallinger, Reinhard | - |
dc.contributor.author | Palacios, Òscar | - |
dc.contributor.author | Capdevila Vidal, Mercè | - |
dc.date.accessioned | 2022-02-25T14:51:31Z | - |
dc.date.available | 2022-02-25T14:51:31Z | - |
dc.date.issued | 2021-12-04 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/2445/183539 | - |
dc.description.abstract | Metallothioneins' (MTs) biological function has been a matter of debate since their discovery. The importance to categorize these cysteine-rich proteins with high coordinating capacity into a specific group led to numerous classification proposals. We proposed a classification based on their metal-binding abilities, gradually sorting them from those with high selectivity towards Zn/Cd to those that are Cu-specific. However, the study of the NpeMT1 and NpeMT2isoforms of Nerita peloronta, has put a new perspective on this classification. N. peloronta has been chosen as a representative mollusk to elucidate the metal-binding abilities of Neritimorpha MTs, an order without any MTs characterized recently. Both isoforms have been recombinantly synthesized in cultures supplemented with ZnII, CdII, or CuII, and the purified metal-MT complexes have been thoroughly characterized by spectroscopic and spectrometric methods, leading to results that confirmed that Neritimorpha share Cd-selective MTs with Caenogastropoda and Heterobranchia, solving a so far unresolved question. NpeMTs show high coordinating preferences towards divalent metal ions, although one of them (NpeMT1) shares features with the so-called genuine Zn-thioneins, while the other (NpeMT2) exhibits a higher preference for Cd. The dissimilarities between the two isoforms let a window open to a new proposal of chemical MT classification. | - |
dc.format.extent | 14 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/ijms222313114 | - |
dc.relation.ispartof | International Journal of Molecular Sciences, 2021, vol. 22, p. 13114 | - |
dc.relation.uri | https://doi.org/10.3390/ijms222313114 | - |
dc.rights | cc-by (c) García-Risco, Mario et al., 2021 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Gastròpodes | - |
dc.subject.classification | Cisteïna | - |
dc.subject.other | Gastropods | - |
dc.subject.other | Cysteine | - |
dc.title | Metal-Specificity Divergence between Metallothioneins of Nerita peloronta (Neritimorpha, Gastropoda) Sets the Starting Point for a Novel Chemical MT Classification Proposal | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 719120 | - |
dc.date.updated | 2022-02-25T14:51:31Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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719120.pdf | 2.06 MB | Adobe PDF | View/Open |
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