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http://hdl.handle.net/2445/184112
Title: | Novel Long-Term Phytase from Serratia odorifera: Cloning, Expression, and Characterization. |
Author: | Salaet, Ignasi Marquès, Ramon Yance Chávez, Tula del Carmen Macías Vidal, Judit Giménez Zaragoza, David Aligué i Alemany, Rosa Maria |
Keywords: | Clonatge Biotecnologia alimentària Cloning Food biotechnology |
Issue Date: | 22-Apr-2021 |
Publisher: | Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
Abstract: | The appA-So gene, encoding a phytase from Serratia odorifera, was cloned and heterologously expressed in Komagataella phaffii. The open reading frame of appA-So comprised 1281 bp that encoded a 426-amino acid protein, including a 27-amino acid signal peptide. The encoded phytase, AppA-So, showed 52% homology with other histidine acid phosphatases. The purified recombinant phytase showed optimal activity at 55 °C and pH 4.5, exhibiting enzymatic activity between pH 3.7 and 5.8, with a specific activity of 1123 U/mg at pH 4.5 and 37 °C. The AppA-So protein retained more than 85% of its initial activity after incubation in different pH conditions (pH 2.5-6.5) at 37 °C for 3 h. AppA-So activity was maintained over time and displayed a low Michaelis-Menten constant (Km) of 0.093 g/L. To the best of our knowledge, this is the first report of the cloning and characterization of the phytase from S. odorifera. Comparison of AppA-So with other well-known phytases suggests that the S. odorifera phytase has the lowest Km and highest stable activity over time, making it very suitable for use in the animal feed industry. |
Note: | Reproducció del document publicat a: https://doi.org/10.1021/acsfoodscitech.0c00074 |
It is part of: | Food Science and Technology, 2021, vol. 1, p. 689-697 |
URI: | http://hdl.handle.net/2445/184112 |
ISSN: | 0101-2061 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
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721487.pdf | 3.64 MB | Adobe PDF | View/Open |
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