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http://hdl.handle.net/2445/184321
Title: | Engineered microtissues for the bystander therapy against cancer |
Author: | Blanco Fernández, Bárbara Garrido, Cristina Rubí Sans, Gerard Sánchez Cid, Lourdes Guerra Rebollo, Marta Rubio Vidal, Núria Mateos Timoneda, Miguel Ángel Engel, Elisabeth Pérez Amodio, Soledad |
Keywords: | Nanomedicina Càncer Nanomedicine Cancer |
Issue Date: | 1-Feb-2021 |
Publisher: | Elsevier |
Abstract: | Thymidine kinase expressing human adipose mesenchymal stem cells (TK-hAMSCs) in combination with ganciclovir (GCV) are an effective platform for antitumor bystander therapy in mice models. However, this strategy requires multiple TK-hAMSCs administrations and a substantial number of cells. Therefore, for clinical translation, it is necessary to find a biocompatible scaffold providing TK-hAMSCs retention in the implantation site against their rapid wash-out. We have developed a microtissue (MT) composed by TKhAMSCs and a scaffold made of polylactic acid microparticles and cell-derived extracellular matrix deposited by hAMSCs. The efficacy of these MTs as vehicles for TK-hAMSCs/GCV bystander therapy was evaluated in a rodent model of human prostate cancer. Subcutaneously implanted MTs were integrated in the surrounding tissue, allowing neovascularization and maintenance of TK-hAMSCs viability. Furthermore, MTs implanted beside tumors allowed TK-hAMSCs migration towards tumor cells and, after GCV administration, inhibited tumor growth. These results indicate that TK-hAMSCs-MTs are promising cell reservoirs for clinical use of therapeutic MSCs in bystander therapies. |
Note: | Reproducció del postprint publicat a: https://doi.org/10.1016/j.msec.2020.111854 |
It is part of: | Materials Science & Engineering C-Materials For Biological Applications, 2021, vol 121 |
URI: | http://hdl.handle.net/2445/184321 |
Related resource: | https://doi.org/10.1016/j.msec.2020.111854 |
ISSN: | 0928-4931 |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
File | Description | Size | Format | |
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2021_MatSciEngC_EngineeredMicro_Engel_Postprint.pdf | 1.58 MB | Adobe PDF | View/Open |
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