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http://hdl.handle.net/2445/184760
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DC Field | Value | Language |
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dc.contributor.author | Albini, Sonia | - |
dc.contributor.author | Coutinho, Paula | - |
dc.contributor.author | Malecova, Barbora | - |
dc.contributor.author | Giordani, Lorenzo | - |
dc.contributor.author | Savchenko, Alex | - |
dc.contributor.author | Forcales Fernàndez, Sonia-Vanina | - |
dc.contributor.author | Puri, Pier Lorenzo | - |
dc.date.accessioned | 2022-04-05T17:42:55Z | - |
dc.date.available | 2022-04-05T17:42:55Z | - |
dc.date.issued | 2013-03-01 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | http://hdl.handle.net/2445/184760 | - |
dc.description.abstract | Direct generation of a homogeneous population of skeletal myoblasts from human embryonic stem cells (hESCs) and formation of three-dimensional contractile structures for disease modeling in vitro are current challenges in regenerative medicine. Previous studies reported on the generation of myoblasts from ESC-derived embryoid bodies (EB), but not from undifferentiated ESCs, indicating the requirement for mesodermal transition to promote skeletal myogenesis. Here, we show that selective absence of the SWI/SNF component BAF60C (encoded by SMARCD3) confers on hESCs resistance to MyoD-mediated activation of skeletal myogenesis. Forced expression of BAF60C enables MyoD to directly activate skeletal myogenesis in hESCs by instructing MyoD positioning and allowing chromatin remodeling at target genes. BAF60C/MyoD-expressing hESCs are epigenetically committed myogenic progenitors, which bypass the mesodermal requirement and, when cultured as floating clusters, give rise to contractile three-dimensional myospheres composed of skeletal myotubes. These results identify BAF60C as a key epigenetic determinant of hESC commitment to the myogenic lineage and establish the molecular basis for the generation of hESC-derived myospheres exploitable for 'disease in a dish' models of muscular physiology and dysfunction. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.celrep.2013.02.012 | - |
dc.relation.ispartof | Cell Reports, 2013, vol. 3, num. 3, p. 661-670 | - |
dc.relation.uri | https://doi.org/10.1016/j.celrep.2013.02.012 | - |
dc.rights | cc-by (c) Albini, Sonia et al., 2013 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Epigenètica | - |
dc.subject.classification | Cèl·lules mare embrionàries | - |
dc.subject.classification | Cèl·lules musculars | - |
dc.subject.classification | Citologia | - |
dc.subject.classification | Transcripció genètica | - |
dc.subject.other | Epigenetics | - |
dc.subject.other | Embryonic stem cells | - |
dc.subject.other | Muscle cells | - |
dc.subject.other | Cytology | - |
dc.subject.other | Genetic transcription | - |
dc.title | Epigenetic reprogramming of human embryonic stem cells into skeletal muscle cells and generation of contractile myospheres | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 684886 | - |
dc.date.updated | 2022-04-05T17:42:55Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/241440/EU//ENDOSTEM | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 23478022 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
File | Description | Size | Format | |
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684886.pdf | 1.89 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License