Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/184760
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dc.contributor.authorAlbini, Sonia-
dc.contributor.authorCoutinho, Paula-
dc.contributor.authorMalecova, Barbora-
dc.contributor.authorGiordani, Lorenzo-
dc.contributor.authorSavchenko, Alex-
dc.contributor.authorForcales Fernàndez, Sonia-Vanina-
dc.contributor.authorPuri, Pier Lorenzo-
dc.date.accessioned2022-04-05T17:42:55Z-
dc.date.available2022-04-05T17:42:55Z-
dc.date.issued2013-03-01-
dc.identifier.issn2211-1247-
dc.identifier.urihttp://hdl.handle.net/2445/184760-
dc.description.abstractDirect generation of a homogeneous population of skeletal myoblasts from human embryonic stem cells (hESCs) and formation of three-dimensional contractile structures for disease modeling in vitro are current challenges in regenerative medicine. Previous studies reported on the generation of myoblasts from ESC-derived embryoid bodies (EB), but not from undifferentiated ESCs, indicating the requirement for mesodermal transition to promote skeletal myogenesis. Here, we show that selective absence of the SWI/SNF component BAF60C (encoded by SMARCD3) confers on hESCs resistance to MyoD-mediated activation of skeletal myogenesis. Forced expression of BAF60C enables MyoD to directly activate skeletal myogenesis in hESCs by instructing MyoD positioning and allowing chromatin remodeling at target genes. BAF60C/MyoD-expressing hESCs are epigenetically committed myogenic progenitors, which bypass the mesodermal requirement and, when cultured as floating clusters, give rise to contractile three-dimensional myospheres composed of skeletal myotubes. These results identify BAF60C as a key epigenetic determinant of hESC commitment to the myogenic lineage and establish the molecular basis for the generation of hESC-derived myospheres exploitable for 'disease in a dish' models of muscular physiology and dysfunction.-
dc.format.extent10 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1016/j.celrep.2013.02.012-
dc.relation.ispartofCell Reports, 2013, vol. 3, num. 3, p. 661-670-
dc.relation.urihttps://doi.org/10.1016/j.celrep.2013.02.012-
dc.rightscc-by (c) Albini, Sonia et al., 2013-
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.sourceArticles publicats en revistes (Patologia i Terapèutica Experimental)-
dc.subject.classificationEpigenètica-
dc.subject.classificationCèl·lules mare embrionàries-
dc.subject.classificationCèl·lules musculars-
dc.subject.classificationCitologia-
dc.subject.classificationTranscripció genètica-
dc.subject.otherEpigenetics-
dc.subject.otherEmbryonic stem cells-
dc.subject.otherMuscle cells-
dc.subject.otherCytology-
dc.subject.otherGenetic transcription-
dc.titleEpigenetic reprogramming of human embryonic stem cells into skeletal muscle cells and generation of contractile myospheres-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec684886-
dc.date.updated2022-04-05T17:42:55Z-
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/241440/EU//ENDOSTEM-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.pmid23478022-
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Publicacions de projectes de recerca finançats per la UE

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