Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/184911
Title: | SARS-CoV-2 RNA and antibody detection in human milk from a prospective multicenter study in Spain |
Author: | Bäuerl, Christine Randazzo, Walter Sánchez, Gloria Selma-Royo, Marta Garcia-Verdevio, Elia Martínez Rodríguez, Laura Parra-Llorca, Anna Lerin, Carles Fumadó, Victoria Crovetto, Francesca Crispi Brillas, Fàtima Pérez-Cano, Francisco J. Rodríguez, Gerardo Ruíz-Redondo, Gema Campoy, Cristina Martínez Costa, Cecilia Collado, Maria Carmen MilkCORONA study team |
Keywords: | Llet materna COVID-19 SARS-CoV-2 Sistema immunitari Breast milk COVID-19 SARS-CoV-2 Immune system |
Issue Date: | 20-Aug-2021 |
Publisher: | BMJ Publishing Group |
Abstract: | Objectives To develop and validate a specific protocol for SARS-CoV-2 detection in breast milk matrix and to determine the impact of maternal SARS-CoV-2 infection on the presence, concentration and persistence of specific SARS-CoV-2 antibodies. Design and patients This is a prospective, multicentre longitudinal study (April-December 2020) in 60 mothers with SARS-CoV-2 infection and/or who have recovered from COVID-19. A control group of 13 women before the pandemic were also included. Setting Seven health centres from different provinces in Spain. Main outcome measures Presence of SARS-CoV-2 RNA in breast milk, targeting the N1 region of the nucleocapsid gene and the envelope (E) gene; presence and levels of SARS-CoV-2-specific immunoglobulins (Igs)¿IgA, IgG and IgM¿in breast milk samples from patients with COVID-19. Results All breast milk samples showed negative results for presence of SARS-CoV-2 RNA. We observed high intraindividual and interindividual variability in the antibody response to the receptor-binding domain of the SARS-CoV-2 spike protein for each of the three isotypes IgA, IgM and IgG. Main Protease (MPro) domain antibodies were also detected in milk. 82.9% (58 of 70) of milk samples were positive for at least one of the three antibody isotypes, with 52.9% of these positive for all three Igs. Positivity rate for IgA was relatively stable over time (65.2%-87.5%), whereas it raised continuously for IgG (from 47.8% for the first 10 days to 87.5% from day 41 up to day 206 post-PCR confirmation). Conclusions Our study confirms the safety of breast feeding and highlights the relevance of virus-specific SARS-CoV-2 antibody transfer. This study provides crucial data to support official breastfeeding recommendations based on scientific evidence. |
Note: | Reproducció del document publicat a: https://doi.org/10.1136/archdischild-2021-322463 |
It is part of: | Archives of Disease in Childhood: Fetal & Neonatal, 2021, vol. 107, num. 2, p. 216-221 |
URI: | http://hdl.handle.net/2445/184911 |
Related resource: | https://doi.org/10.1136/archdischild-2021-322463 |
ISSN: | 1359-2998 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) Articles publicats en revistes (BCNatal Fetal Medicine Research Center) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
721527.pdf | 376.99 kB | Adobe PDF | View/Open |
This item is licensed under a
Creative Commons License