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Title: SARS-CoV-2 RNA and antibody detection in human milk from a prospective multicenter study in Spain
Author: Bäuerl, Christine
Randazzo, Walter
Sánchez, Gloria
Selma-Royo, Marta
Garcia-Verdevio, Elia
Martínez Rodríguez, Laura
Parra-Llorca, Anna
Lerin, Carles
Fumadó, Victoria
Crovetto, Francesca
Crispi Brillas, Fàtima
Pérez-Cano, Francisco J.
Rodríguez, Gerardo
Ruíz-Redondo, Gema
Campoy, Cristina
Martínez Costa, Cecilia
Collado, Maria Carmen
MilkCORONA study team
Keywords: Llet materna
Sistema immunitari
Breast milk
Immune system
Issue Date: 20-Aug-2021
Publisher: BMJ Publishing Group
Abstract: Objectives To develop and validate a specific protocol for SARS-CoV-2 detection in breast milk matrix and to determine the impact of maternal SARS-CoV-2 infection on the presence, concentration and persistence of specific SARS-CoV-2 antibodies. Design and patients This is a prospective, multicentre longitudinal study (April-December 2020) in 60 mothers with SARS-CoV-2 infection and/or who have recovered from COVID-19. A control group of 13 women before the pandemic were also included. Setting Seven health centres from different provinces in Spain. Main outcome measures Presence of SARS-CoV-2 RNA in breast milk, targeting the N1 region of the nucleocapsid gene and the envelope (E) gene; presence and levels of SARS-CoV-2-specific immunoglobulins (Igs)¿IgA, IgG and IgM¿in breast milk samples from patients with COVID-19. Results All breast milk samples showed negative results for presence of SARS-CoV-2 RNA. We observed high intraindividual and interindividual variability in the antibody response to the receptor-binding domain of the SARS-CoV-2 spike protein for each of the three isotypes IgA, IgM and IgG. Main Protease (MPro) domain antibodies were also detected in milk. 82.9% (58 of 70) of milk samples were positive for at least one of the three antibody isotypes, with 52.9% of these positive for all three Igs. Positivity rate for IgA was relatively stable over time (65.2%-87.5%), whereas it raised continuously for IgG (from 47.8% for the first 10 days to 87.5% from day 41 up to day 206 post-PCR confirmation). Conclusions Our study confirms the safety of breast feeding and highlights the relevance of virus-specific SARS-CoV-2 antibody transfer. This study provides crucial data to support official breastfeeding recommendations based on scientific evidence.
Note: Reproducció del document publicat a:
It is part of: Archives of Disease in Childhood: Fetal & Neonatal, 2021, vol. 107, num. 2, p. 216-221
Related resource:
ISSN: 1359-2998
Appears in Collections:Articles publicats en revistes (BCNatal Fetal Medicine Research Center)
Articles publicats en revistes (Bioquímica i Fisiologia)

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