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Title: Development of DNA Aptamers Against <i>Plasmodium falciparum</i> Blood Stages Using Cell-Systematic Evolution of Ligands by EXponential Enrichment.
Author: Lantero, Elena
Belavilas Trovas, Alexandros
Biosca, Arnau
Recolons, Paula
Moles Meler, Ernest
Sulleiro, Elena
Zarzuela, Francesc
Avalos Padilla, Yunuen
Ramírez, Míriam
Fernàndez Busquets, Xavier
Keywords: Malària
Issue Date: 1-Mar-2020
Publisher: American Scientific Publishers
Abstract: "New biomarkers have to be developed in order to increase the performance of current antigen-based malaria rapid diagnosis. Antibody production often involves the use of laboratory animals and is time-consuming and costly, especially when the target is " - ", whose variable antigen expression complicates the development of long-lived biomarkers. To circumvent these obstacles, we have applied the Systematic Evolution of Ligands by EXponential enrichment method to the rapid identification of DNA aptamers against " - "-infected red blood cells (pRBCs). Five 70 b-long ssDNA sequences, and their shorter forms without the flanking PCR primer-binding regions, have been identified having a highly specific binding of pRBCs versus non-infected erythrocytes. Structural analysis revealed G-enriched sequences compatible with the formation of G-quadruplexes. The selected aptamers recognized intracellular epitopes with apparent " - "s in the " - "M range in both fixed and non-fixed saponin-permeabilized pRBCs, improving >30-fold the pRBC detection in comparison with aptamers raised against " - " lactate dehydrogenase, the gold standard antigen for current malaria diagnostic tests. In thin blood smears of clinical samples the aptamers reported in this work specifically bound all " - " stages versus non-infected erythrocytes, and also detected early and late stages of the human malaria parasites " - ", " - " and " - . The results are discussed in the context of their potential application in future malaria diagnostic devices.
Note: Postprint del document publicat a:
It is part of: Journal of biomedical nanotechnology, 2020, vol. 16, num. 3, p. 315-334
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ISSN: 1550-7033
Appears in Collections:Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (ISGlobal)
Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB))

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