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http://hdl.handle.net/2445/185018
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DC Field | Value | Language |
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dc.contributor.author | Lantero, Elena | - |
dc.contributor.author | Belavilas Trovas, Alexandros | - |
dc.contributor.author | Biosca, Arnau | - |
dc.contributor.author | Recolons, Paula | - |
dc.contributor.author | Moles Meler, Ernest | - |
dc.contributor.author | Sulleiro, Elena | - |
dc.contributor.author | Zarzuela, Francesc | - |
dc.contributor.author | Avalos Padilla, Yunuen | - |
dc.contributor.author | Ramírez, Míriam | - |
dc.contributor.author | Fernàndez Busquets, Xavier | - |
dc.date.accessioned | 2022-04-19T12:25:10Z | - |
dc.date.available | 2022-04-19T12:25:10Z | - |
dc.date.issued | 2020-03-01 | - |
dc.identifier.issn | 1550-7033 | - |
dc.identifier.uri | http://hdl.handle.net/2445/185018 | - |
dc.description.abstract | "New biomarkers have to be developed in order to increase the performance of current antigen-based malaria rapid diagnosis. Antibody production often involves the use of laboratory animals and is time-consuming and costly, especially when the target is " - ", whose variable antigen expression complicates the development of long-lived biomarkers. To circumvent these obstacles, we have applied the Systematic Evolution of Ligands by EXponential enrichment method to the rapid identification of DNA aptamers against " - "-infected red blood cells (pRBCs). Five 70 b-long ssDNA sequences, and their shorter forms without the flanking PCR primer-binding regions, have been identified having a highly specific binding of pRBCs versus non-infected erythrocytes. Structural analysis revealed G-enriched sequences compatible with the formation of G-quadruplexes. The selected aptamers recognized intracellular epitopes with apparent " - "s in the " - "M range in both fixed and non-fixed saponin-permeabilized pRBCs, improving >30-fold the pRBC detection in comparison with aptamers raised against " - " lactate dehydrogenase, the gold standard antigen for current malaria diagnostic tests. In thin blood smears of clinical samples the aptamers reported in this work specifically bound all " - " stages versus non-infected erythrocytes, and also detected early and late stages of the human malaria parasites " - ", " - " and " - . The results are discussed in the context of their potential application in future malaria diagnostic devices. | - |
dc.format.extent | 20 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Scientific Publishers | - |
dc.relation.isformatof | Postprint del document publicat a: http://dx.doi.org/10.1166/jbn.2020.2901 | - |
dc.relation.ispartof | Journal of biomedical nanotechnology, 2020, vol. 16, num. 3, p. 315-334 | - |
dc.relation.uri | http://dx.doi.org/10.1166/jbn.2020.2901 | - |
dc.rights | (c) American Scientific Publishers, 2020 | - |
dc.source | Articles publicats en revistes (ISGlobal) | - |
dc.subject.classification | Malària | - |
dc.subject.classification | Nanotecnologia | - |
dc.subject.other | Malaria | - |
dc.subject.other | Nanotechnology | - |
dc.title | Development of DNA Aptamers Against <i>Plasmodium falciparum</i> Blood Stages Using Cell-Systematic Evolution of Ligands by EXponential Enrichment. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.date.updated | 2022-04-08T18:00:43Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 32493542 | - |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Articles publicats en revistes (ISGlobal) Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB)) |
Files in This Item:
File | Description | Size | Format | |
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Lantero_E_J_Biomed_Nanotechnol_2020_Postprint.pdf | 776.57 kB | Adobe PDF | View/Open |
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