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http://hdl.handle.net/2445/185018
Title: | Development of DNA Aptamers Against <i>Plasmodium falciparum</i> Blood Stages Using Cell-Systematic Evolution of Ligands by EXponential Enrichment. |
Author: | Lantero, Elena Belavilas Trovas, Alexandros Biosca, Arnau Recolons, Paula Moles Meler, Ernest Sulleiro, Elena Zarzuela, Francesc Avalos Padilla, Yunuen Ramírez, Míriam Fernàndez Busquets, Xavier |
Keywords: | Malària Nanotecnologia Malaria Nanotechnology |
Issue Date: | 1-Mar-2020 |
Publisher: | American Scientific Publishers |
Abstract: | "New biomarkers have to be developed in order to increase the performance of current antigen-based malaria rapid diagnosis. Antibody production often involves the use of laboratory animals and is time-consuming and costly, especially when the target is " - ", whose variable antigen expression complicates the development of long-lived biomarkers. To circumvent these obstacles, we have applied the Systematic Evolution of Ligands by EXponential enrichment method to the rapid identification of DNA aptamers against " - "-infected red blood cells (pRBCs). Five 70 b-long ssDNA sequences, and their shorter forms without the flanking PCR primer-binding regions, have been identified having a highly specific binding of pRBCs versus non-infected erythrocytes. Structural analysis revealed G-enriched sequences compatible with the formation of G-quadruplexes. The selected aptamers recognized intracellular epitopes with apparent " - "s in the " - "M range in both fixed and non-fixed saponin-permeabilized pRBCs, improving >30-fold the pRBC detection in comparison with aptamers raised against " - " lactate dehydrogenase, the gold standard antigen for current malaria diagnostic tests. In thin blood smears of clinical samples the aptamers reported in this work specifically bound all " - " stages versus non-infected erythrocytes, and also detected early and late stages of the human malaria parasites " - ", " - " and " - . The results are discussed in the context of their potential application in future malaria diagnostic devices. |
Note: | Postprint del document publicat a: http://dx.doi.org/10.1166/jbn.2020.2901 |
It is part of: | Journal of biomedical nanotechnology, 2020, vol. 16, num. 3, p. 315-334 |
URI: | http://hdl.handle.net/2445/185018 |
Related resource: | http://dx.doi.org/10.1166/jbn.2020.2901 |
ISSN: | 1550-7033 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB)) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
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File | Description | Size | Format | |
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Lantero_E_J_Biomed_Nanotechnol_2020_Postprint.pdf | 776.57 kB | Adobe PDF | View/Open |
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