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Title: | High within-host diversity found from direct genotyping on post-mortem tuberculosis specimens in a high-burden setting |
Author: | Rodríguez Grande, Cristina Hurtado, Juan Carlos Rodríguez Maus, Sandra Casas, Isaac Castillo, Paola Navarro, Mireia Rakislova, Natalia García-Basteiro, Alberto L. Carrilho, Carla Fernandes, Fabiola Lovane, Lucilia Jordao, Dercio Ismail, Mamudo Rafik Lorenzoni, Cesaltina Cossa, Anelsio Mandomando, Inácio Bassat Orellana, Quique Menéndez, Clara Ordi i Majà, Jaume Muñoz, Patricia Pérez Lago, Laura García de Viedma, Darío Martínez Yoldi, Miguel Julián |
Keywords: | Mycobacterium tuberculosis Àfrica oriental Genètica bacteriana Autòpsia Anàlisi Mycobacterium tuberculosis East Africa Bacterial genetics Autopsy Assaying |
Issue Date: | 11-Jun-2021 |
Publisher: | European Society of Clinical Microbiology and Infectious Diseases |
Abstract: | Objectives: To characterize the clonal complexity in Mycobacterium tuberculosis (MTB) infections considering factors that help maximize the detection of coexisting strains/variants. Methods: Genotypic analysis by Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeats (MIRU-VNTR) was performed directly on 70 biopsy specimens from two or more different tissues involving 28 tuberculosis cases diagnosed post-mortem in Mozambique, a country with a high tuberculosis burden. Results: Genotypic data from isolates collected from two or more tissues were obtained for 23 of the 28 cases (82.1%), allowing the analysis of within-patient diversity. MIRU-VNTR analysis revealed clonal diversity in ten cases (35.7%). Five cases showed allelic differences in three or more loci, suggesting mixed infection with two different strains. In half of the cases showing within-host diversity, one of the specimens associated with clonal heterogeneity was brain tissue. Conclusions: Direct MTB genotyping from post-mortem tissue samples revealed a frequent within-host Mycobacterium tuberculosis diversity, including mixed and polyclonal infections. Most of this diversity would have been overlooked if only standard analysis of respiratory specimens had been performed. |
Note: | Reproduccío del document publicat a: https://doi.org/10.1016/j.cmi.2021.05.038 |
It is part of: | Clinical Microbiology and Infection, 2021, vol. 27, num. 10, p. 1518 |
URI: | http://hdl.handle.net/2445/185442 |
Related resource: | https://doi.org/10.1016/j.cmi.2021.05.038 |
ISSN: | 1198-743X |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) |
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