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Title: Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
Author: Mompeon, Ana
Ortega Paz, Luis
Vidal Gomez, Xavier
Januario Costa, Tiago
Perez Cremades, Daniel
Garcia Blas, Sergio
Brugaletta, Salvatore
Sanchis Forés, Juan
Sabaté Tenas, Manuel
Novella, Susana
Dantas, Ana Paula
Hermenegildo, Carlos
Keywords: Vellesa
Micro RNAs
Infart de miocardi
Plasma sanguini
Old age
Myocardial infarction
Blood plasma
Issue Date: 25-Mar-2020
Publisher: Nature Publishing Group
Abstract: Despite the promising value of miRNAs in the diagnostic and prognostic of cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances in studies may interfere with miRNA profle and afect their results. This study determines if the blood starting material is a source of variance in miRNA profle by performing a paired comparison in plasma and serum of the expression of primary miRNAs associated with CVD. Circulating miRNA yield was similar in both plasma and serum, although a signifcant increase was observed in patients with Non-ST-elevation myocardial infarction (NSTEMI) compared to control volunteers. When normalized by the expression of miR-484, diferent patterns of miRNA expression between serum and plasma. Although NSTEMI modifed the expression of miR-1 and miR-208 in both serum and plasma, plasma displayed a higher variance than serum (Levene's test p<0.01). For miR-133a and miR-26a, diferences were only detected in serum (p=0.0240), and conversely, miR-499a showed diferences only in plasma of NSTEMI (p=0.001). Interestingly, miR-21 showed an opposite pattern of expression, being increased in serum (2−ΔΔCt : 5.7, p=0.0221) and decreased in plasma (2−ΔΔCt : 0.5, p=0.0107). Plasma and serum exhibit diferent patterns of circulating miRNA expression in NSTEMI and suggest that results from studies with diferent starting material could not be comparable.
Note: Reproducció del document publicat a:
It is part of: Scientific Reports, 2020, vol. 10, num. 1, p. 5373
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (Medicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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