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http://hdl.handle.net/2445/189205
Title: | Hypoxia inducible factor-1α accumulation in steatotic liver preservation: role of nitric oxide |
Author: | Zaouali, Mohamed Amine Ben Mosbah, Ismail Boncompagni, Eleonora Ben Abdennebi, Hassen Mitjavila Cors, Maria Teresa Bartrons Bach, Ramon Freitas, Isabel Rimola Castellá, Antonio Roselló Catafau, Juan |
Keywords: | Malalties del fetge Òxid nítric Oxigen en l'organisme Conservació d'òrgans Liver diseases Nitric oxide Oxygen in the body Preservation of organs |
Issue Date: | 2010 |
Publisher: | Baishideng Publishing Group Inc |
Abstract: | AIM: To examine the relevance of hypoxia inducible factor (HIF-1) and nitric oxide (NO) on the preservation of fatty liver against cold ischemia-reperfusion injury (IRI). METHODS: We used an isolated perfused rat liver model and we evaluated HIF-1α in steatotic and non-steatotic livers preserved for 24 h at 4°C in University of Wisconsin and IGL-1 solutions, and then subjected to 2 h of normothermic reperfusion. After normoxic reperfusion, liver enzymes, bile production, bromosulfophthalein clearance, as well as HIF-1α and NO [endothelial NO synthase (eNOS) activity and nitrites/nitrates] were also measured. Other factors associated with the higher susceptibility of steatotic livers to IRI, such as mitochondrial damage and vascular resistance were evaluated. RESULTS: A significant increase in HIF-1α was found in steatotic and non-steatotic livers preserved in IGL-1 after cold storage. Livers preserved in IGL-1 showed a significant attenuation of liver injury and improvement in liver function parameters. These benefits were enhanced by the addition of trimetazidine (an anti-ischemic drug), which induces NO and eNOS activation, to IGL-1 solution. In normoxic reperfusion, the presence of NO favors HIF-1α accumulation, promoting also the activation of other cytoprotective genes, such as heme-oxygenase-1. CONCLUSION: We found evidence for the role of the HIF-1α/NO system in fatty liver preservation, especially when IGL-1 solution is used. |
Note: | Reproducció del document publicat a: https://doi.org/10.3748/wjg.v16.i28.3499 |
It is part of: | World Journal of Gastroenterology, 2010, vol. 16, num. 28, p. 3499-3509 |
URI: | http://hdl.handle.net/2445/189205 |
Related resource: | https://doi.org/10.3748/wjg.v16.i28.3499 |
ISSN: | 1007-9327 |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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