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Title: Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents
Author: Laveriano-Santos, Emily P.
Marhuenda-Muñoz, María
Vallverdú i Queralt, Anna
Martínez Huélamo, Miriam
Tresserra i Rimbau, Anna
Miliarakis, Eleftherios
Arancibia Riveros, Camila
Jáuregui Pallarés, Olga
Ruíz León, Ana María
Castro-Baquero, Sara
Estruch, Ramon
Bodega, Patricia
Miguel, Mercedes de
Cos Gandoy, Amaya de
Martínez Gomez, Jesús
Santos-Beneit, Gloria
Fernández Alvira, Juan Miguel
Fernández Jiménez, Rodrigo
Lamuela Raventós, Rosa Ma.
Keywords: Microbiota intestinal
Gastrointestinal microbiome
Issue Date: Jun-2022
Publisher: MDPI
Abstract: This study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relationship between MPM and dietary (poly)phenols in Spanish adolescents. A total of 601 spot urine samples of adolescents aged 12.02 ± 0.41 years were analyzed. The quantitative method was validated for linearity, limit of detection, limit of quantification, recovery, intra-and inter-day accuracy and precision, as well as postpreparative stability according to the criteria established by the Association of Official Agricultural Chemists International. A total of 17 aglycones and 37 phase II MPM were identified and quantified in 601 spot urine samples. Phenolic acids were the most abundant urinary MPM, whereas stilbenes, hydroxytyrosol, and enterodiol were the least abundant. Urinary hydroxycoumarin acids (urolithins) were positively correlated with flavonoid and total (poly)phenol intake. An HPLC-ESI-LTQ-Orbitrap-HRMS method was developed and fully validated to quantify MPM. The new method was performed accurately and is suitable for MPM quantification in large epidemiological studies. Urinary lignans and urolithins are proposed as potential biomarkers of grain and nut intake in an adolescent population.
Note: Reproducció del document publicat a:
It is part of: Antioxidants, 2022, vol. 11, num. 6, p. 1167
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ISSN: 2076-3921
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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