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Title: Separation and characterization of bovine milk proteins by capillary electrophoresis-mass spectrometry
Author: Ghafoori, Zahra
Tehrani, Tahereh
Pont Villanueva, Laura
Benavente Moreno, Fernando J. (Julián)
Keywords: Proteïnes de la llet
Electroforesi capil·lar
Espectrometria de masses
Milk proteins
Capillary electrophoresis
Mass spectrometry
Issue Date: 2022
Publisher: Wiley-VCH
Abstract: Protein profiling of major bovine milk proteins (i.e. whey and casein proteins) is of great interest in food science and technology. This complex set of protein proteoforms may vary with breed, genetics, lactation stage, health and nutritional status of the animal. Current routine methods for bovine milk protein profiling at the intact level are typically based on CE-UV, which does not allow confirming unequivocally the identity of the separated proteins. As an alternative, in this study, we describe for the first time a novel and simple CE-MS method in positive ESI mode. Under the optimized conditions, CE-MS allowed the separation and identification at the intact level of major bovine milk whey and casein proteins in less than 15 min. Furthermore, high-resolution MS confirmed its importance in the reliable characterization of bovine milk protein proteoforms, especially those with slight molecular mass differences, such as β-casein A1 and A2, which are relevant to unequivocally identify milks with specific β-casein compositions (e.g. A2A2 milks, which are widely known as A2 milks). This differentiation was not possible by MALDI-MS, which provided rapidly and easily a rich but less accurate fingerprint of bovine milk proteins due to the lower mass resolution.
Note: Reproducció del document publicat a:
It is part of: Journal of Separation Science, 2022, vol. 45, num. 18, p. 1-10
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ISSN: 1615-9306
Appears in Collections:Articles publicats en revistes (Institut de Recerca en Nutrició i Seguretat Alimentària (INSA·UB))
Articles publicats en revistes (Enginyeria Química i Química Analítica)

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