Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/192288
Title: | The SARS-CoV-2 spike protein binds and modulates estrogen receptors |
Author: | Solis, Oscar Beccari, Andrea R. Iaconis, Daniela Talarico, Carmine Ruiz Bedoya, Camilo A. Nwachukwu, Jerome C. Cimini, Annamaria Castelli, Vanessa Bertini, Riccardo Montopoli, Monica Cocetta, Veronica Borocci, Stefano Prandi, Ingrid G. Flavahan, Kelly Bahr, Melissa Napiorkowski, Anna Chillemi, Giovanni Ooka, Masato Yang, Xiaoping Zhang, Shiliang Xia, Menghang Zheng, Wei Bonaventura, Jordi Pomper, Martin G. Hooper, Jody E. Morales, Marisela Rosenberg, Avi Z. Nettles, Kendall W. Jain, Sanjay K. Allegretti, Marcello Michaelides, Michael |
Keywords: | SARS-CoV-2 Pandèmia de COVID-19, 2020- Fixació de proteïnes SARS-CoV-2 COVID-19 Pandemic, 2020- Protein binding |
Issue Date: | 2-Dec-2022 |
Publisher: | American Association for the Advancement of Science (AAAS) |
Abstract: | The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein binds angiotensin-converting enzyme 2 as its primary infection mechanism. Interactions between S and endogenous proteins occur after infection but are not well understood. We profiled binding of S against >9000 human proteins and found an interaction between S and human estrogen receptor alpha (ER alpha). Using bioinformatics, supercomputing, and experimental assays, we identified a highly conserved and functional nuclear receptor coregulator (NRC) LXD-like motif on the S2 sub-unit. In cultured cells, S DNA transfection increased ER alpha cytoplasmic accumulation, and S treatment induced ER-dependent biological effects. Non-invasive imaging in SARS-CoV-2-infected hamsters localized lung pathology with increased ER alpha lung levels. Postmortem lung experiments from infected hamsters and humans confirmed an increase in cytoplasmic ER alpha and its colocalization with S in alveolar macrophages. These findings describe the discovery of a S-ER alpha interaction, imply a role for S as an NRC, and advance knowledge of SARS-CoV-2 biology and coronavirus disease 2019 pathology. |
Note: | Reproducció del document publicat a: https://doi.org/10.1126/sciadv.add4150 |
It is part of: | Science Advances, 2022, vol. 8, num. 48, p. eadd4150 |
URI: | http://hdl.handle.net/2445/192288 |
Related resource: | https://doi.org/10.1126/sciadv.add4150 |
ISSN: | 2375-2548 |
Appears in Collections: | Articles publicats en revistes (Institut de Neurociències (UBNeuro)) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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