Please use this identifier to cite or link to this item:
Title: Fusion of the human gene for the polyubiquitination coeffector UEV1 with Kua, a newly identified gene
Author: Thomson, Timothy M.
Lozano, Juan José
Loukili, Noureddine
Carrió, Roberto
Serras Rigalt, Florenci
Cormand Rifà, Bru
Valeri, Marta
Díaz, Víctor M.
Abril Ferrando, Josep Francesc, 1970-
Burset Albareda, Moisès
Merino, Jesús
Macaya, Alfons
Corominas, Montserrat (Corominas Guiu)
Guigó, Roderic
Keywords: Mapatge cromosòmic humà
Human gene mapping
Issue Date: 2000
Publisher: Cold Spring Harbor Laboratory Press
Abstract: UEV proteins are enzymatically inactive variants of the E2 ubiquitin-conjugating enzymes that regulate noncanonical elongation of ubiquitin chains. In Saccharomyces cerevisiae, UEV is part of the RAD6-mediated error-free DNA repair pathway. In mammalian cells, UEV proteins can modulate c-FOS transcription and the G2-M transition of the cell cycle. Here we show that the UEV genes from phylogenetically distant organisms present a remarkable conservation in their exon-intron structure. We also show that the human UEV1 gene is fused with the previously unknown geneKua. In Caenorhabditis elegans and Drosophila melanogaster, Kua and UEV are in separated loci, and are expressed as independent transcripts and proteins. In humans,Kua and UEV1 are adjacent genes, expressed either as separate transcripts encoding independent Kua and UEV1 proteins, or as a hybrid Kua-UEV transcript, encoding a two-domain protein. Kua proteins represent a novel class of conserved proteins with juxtamembrane histidine-rich motifs. Experiments with epitope-tagged proteins show that UEV1A is a nuclear protein, whereas both Kua and Kua-UEV localize to cytoplasmic structures, indicating that the Kua domain determines the cytoplasmic localization of Kua-UEV. Therefore, the addition of a Kua domain to UEV in the fused Kua-UEV protein confers new biological properties to this regulator of variant polyubiquitination.
Note: Reproducció del document publicat a:
It is part of: Genome Research, 2000, vol. 10 , num. 11, p. 1743 -1756
Related resource:
ISSN: 1088-9051
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Files in This Item:
File Description SizeFormat 
187133.pdf7.18 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons