Please use this identifier to cite or link to this item:
Title: Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
Author: Carcereny, Albert
Martínez-Velázquez, Adán
Bosch, Albert
Allende, Anna
Truchado, Pilar
Cascales, Jenifer
Romalde, Jesús
Lois, Marta
Polo, David
Sánchez, Gloria
Pérez-Cataluña, Alba
Díaz-Reolid, Azahara
Antón, Andrés
Gregor, Josep
Garcia-Cehic, Damir
Quer, Josep
Palau, Margarita
González Ruano, Cristina
Pintó Solé, Rosa María
Guix, Susana
Keywords: SARS-CoV-2
Issue Date: 2021
Publisher: American Chemical Society
Abstract: Since its first identification in the United Kingdom in late 2020, the highly transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant in several countries raising great concern. We developed a duplex real-time RT-qPCR assay to detect, discriminate, and quantitate SARS-CoV-2 variants containing one of its mutation signatures, the ΔHV69/70 deletion, and used it to trace the community circulation of the B.1.1.7 variant in Spain through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19). The B.1.1.7 variant was detected earlier than clinical epidemiological reporting by the local authorities, first in the southern city of Málaga (Andalucía) in week 20_52 (year_week), and multiple introductions during Christmas holidays were inferred in different parts of the country. Wastewater-based B.1.1.7 tracking showed a good correlation with clinical data and provided information at the local level. Data from wastewater treatment plants, which reached B.1.1.7 prevalences higher than 90% for ≥2 consecutive weeks showed that 8.1 ± 2.0 weeks were required for B.1.1.7 to become dominant. The study highlights the applicability of RT-qPCR-based strategies to track specific mutations of variants of concern as soon as they are identified by clinical sequencing and their integration into existing wastewater surveillance programs, as a cost-effective approach to complement clinical testing during the COVID-19 pandemic.
Note: Versió postprint del document publicat a:
It is part of: Environmental Science & Technology, 2021, vol. 55, num. 17, p. 11756-11766
Related resource:
ISSN: 0013-936X
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Files in This Item:
File Description SizeFormat 
719030.pdf1.23 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.