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http://hdl.handle.net/2445/197645
Title: | The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes |
Author: | Riedelová, Zuzana Santos Pereira, Andres de los Svoboda, Jan Pop Georgievski, Ognen Májek, Pavel Pečánková, Klára Dyčka, Filip Rodriguez Emmenegger, César Riedel, Tomáš |
Keywords: | Trombosi Materials biomèdics Polímers en medicina Thrombosis Biomedical materials Polymers in medicine |
Issue Date: | 2-Aug-2022 |
Publisher: | Wiley |
Abstract: | Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. Thus, considerable efforts are directed towards the preparation of fouling-resistant surfaces with the best possible hemocompatibility. Herein, a comprehensive hemocompatibility study after heparinized blood contact with seven polymer brushes prepared by surface-initiated atom transfer radical polymerization is reported. The resistance to fouling is quantified and thrombus formation and deposition of blood cellular components on the coatings are analyzed. Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. The fouling from undiluted blood plasma is reduced by up to 99%. Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). In addition, several proteins that are not previously linked with blood-biomaterial interaction are presented and discussed. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1002/mabi.202200247 |
It is part of: | Macromolecular Bioscience, 2022, vol. 22, num. 11, p. 2200247 |
URI: | http://hdl.handle.net/2445/197645 |
Related resource: | https://doi.org/10.1002/mabi.202200247 |
ISSN: | 1616-5195 |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
Files in This Item:
File | Description | Size | Format | |
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2022_MacBio_Relation_Riedel_etal_postprint.pdf | 1.12 MB | Adobe PDF | View/Open |
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