Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/197753
Title: | A Mouse Brain-based Multi-omics Integrative Approach Reveals Potential Blood Biomarkers for Ischemic Stroke |
Author: | Simats, Alba Ramiro, Laura Garcia-Berrocoso, Teresa Briansó, Ferran Gonzalo, Ricardo Martín, Luna Sabé, Anna Gill, Natalia Penalba, Anna Colomé, Núria Sànchez, Àlex (Sànchez Pla) Viñals Canals, Francesc Bustamante, Alejandro Rosell, Anna Montaner Villalonga, Joan |
Keywords: | Models animals en la investigació Bioinformàtica Biologia de sistemes Animal models in research Bioinformatics Systems biology |
Issue Date: | 19-Dec-2020 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | Stroke remains a leading cause of death and disability worldwide. Despite continuous advances, the identification of key molecular signatures in the hyper-acute phase of ischemic stroke is still a primary interest for translational research on stroke diagnosis, prognosis, and treatment. Data integration from high-throughput -omics techniques has become crucial to unraveling key interactions among different molecular elements in complex biological contexts, such as ischemic stroke. Thus, we used advanced data integration methods for a multi-level joint analysis of transcriptomics and proteomics data sets obtained from mouse brains at 2 h after cerebral ischemia. By modeling net-like correlation structures, we identified an integrated network of genes and proteins that are differentially expressed at a very early stage after stroke. We validated 10 of these deregulated elements in acute stroke, and changes in their expression pattern over time after cerebral ischemia were described. Of these, CLDN20, GADD45G, RGS2, BAG5, and CTNND2 were next evaluated as blood biomarkers of cerebral ischemia in mice and human blood samples, which were obtained from stroke patients and patients presenting stroke-mimicking conditions. Our findings indicate that CTNND2 levels in blood might potentially be useful for distinguishing ischemic strokes from stroke-mimicking conditions in the hyper-acute phase of the disease. Furthermore, circulating GADD45G content within the first 6 h after stroke could also play a key role in predicting poor outcomes in stroke patients. For the first time, we have used an integrative biostatistical approach to elucidate key molecules in the initial stages of stroke pathophysiology and highlight new notable molecules that might be further considered as blood biomarkers of ischemic stroke. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/mcp.RA120.002283 |
It is part of: | Molecular & Cellular Proteomics, 2020, vol. 19, num. 12, p. 1921-1936 |
URI: | http://hdl.handle.net/2445/197753 |
Related resource: | https://doi.org/10.1074/mcp.RA120.002283 |
ISSN: | 1535-9476 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
709407.pdf | 1.81 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.