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Title: Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance
Author: Comelles Pujadas, Jordi
Fernández Majada, Vanesa
Acevedo, Verónica
Rebollo Calderon, Beatriz
Martínez Fraiz, Elena
Keywords: Migració cel·lular
Cell migration
Issue Date: 27-Jan-2022
Publisher: Elsevier
Abstract: Topographical patterns are a powerful tool to study directional migration. Grooved substrates have been extensively used as in vitro models of aligned extracellular matrix fibers because they induce cell elongation, alignment, and migration through a phenomenon known as contact guidance. This process, which involves the orientation of focal adhesions, F-actin, and microtubule cytoskeleton along the direction of the grooves, has been primarily studied on hard materials of non-physiological stiffness. But how it unfolds when the stiffness of the grooves varies within the physiological range is less known. Here we show that substrate stiffness modulates the cellular response to topographical contact guidance. We find that for fibroblasts, while focal adhesions and actin respond to topography independently of the stiffness, microtubules show a stiffness-dependent response that regulates contact guidance. On the other hand, both clusters and single breast carcinoma epithelial cells display stiffnessdependent contact guidance, leading to more directional and efficient migration when increasing substrate stiffness. These results suggest that both matrix stiffening and alignment of extracellular matrix fibers cooperate during directional cell migration, and that the outcome differs between cell types depending on how they organize their cytoskeletons.
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It is part of: Materials Today Bio, 2022, vol. 19, num. 100593
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ISSN: 2590-0064
Appears in Collections:Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)

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