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Title: | Determination of a lectin protein allergen in food by on-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry |
Author: | Vergara-Barberán, María Simó-Alfonso, Ernesto F. Herrero-Martínez, José Manuel Benavente Moreno, Fernando J. (Julián) |
Keywords: | Espectrometria de masses Electroforesi capil·lar Lectines Mass spectrometry Capillary electrophoresis Lectins |
Issue Date: | 2023 |
Publisher: | Elsevier B.V. |
Abstract: | An aptamer affinity sorbent was prepared for clean-up, preconcentration, separation and characterization of a food allergen protein by on-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry (AA-SPE-CE-MS). SPE microcartridges were packed with a sorbent based on magnetic bead particles modified with an aptamer against the target lectin protein concanavalin A (Con A). After optimization of several parameters of the SPE-CE-MS method, the sample (ca. 30 µL) was loaded in separation background electrolyte (BGE, 2 M acetic acid pH 2.2), while the retained protein was eluted with 100 mM NH4OH (pH 11.2) (ca. 100 nL). The developed method was linear between 0.5 and 20 mg·L-1 and the limit of detection (LOD) was 0.25 mg·L-1, which was 100 times lower than by CE-MS. The repeatability of the method was satisfactory, with relative standard deviations (RSD) for migration times and peak areas below 1.9 and 8.1%, respectively. In addition, the microcartridges could be reused more than 25 analyses without significant loss of extraction efficiency. Finally, the applicability and versatility of the developed method were demonstrated by analyzing low levels of Con A in different food matrices (i.e. white beans, as well as chickpea, lentils, and wheat flours), leading to satisfactory results, with recoveries between 87 and 115%. |
Note: | Reproducció del document publicat a: https://doi.org/10.1016/j.microc.2023.108746 |
It is part of: | Microchemical Journal, 2023, vol. 190, p. 108746 |
URI: | http://hdl.handle.net/2445/199376 |
Related resource: | https://doi.org/10.1016/j.microc.2023.108746 |
ISSN: | 0026-265X |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) Articles publicats en revistes (Institut de Recerca en Nutrició i Seguretat Alimentària (INSA·UB)) |
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