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dc.contributor.authorCanals, Jordi-
dc.contributor.authorNavarro Pons, Alfons-
dc.contributor.authorVila, Cristina-
dc.contributor.authorCanals i Coll, Josep M.-
dc.contributor.authorDíaz, Tania-
dc.contributor.authorAcosta Plasencia, Melissa-
dc.contributor.authorCros Font, Coralí-
dc.contributor.authorHan, Bing-
dc.contributor.authorHe, Yangyi-
dc.contributor.authorMonzó Planella, Mariano-
dc.description.abstractBackground: When genes responsible for normal embryonic development are abnormally expressed in adults, it can lead to tumor development. This can suggest that the same mechanism that controls embryonic differentiation can also control tumor differentiation. We hypothesize that the malignant phenotype of lung cancer cells could acquire benign characteristics when in contact with an embryonic lung microenvironment. We cultured two lung cancer cell lines in embryonic lung mesenchyme-conditioned medium and evaluated morphological, functional and molecular changes. Methods: The human embryonic mesenchymal lung-conditioned medium (hEML-CM) was obtained by culturing lung cells from embryos in the pseudoglandular stage of development. The NSCLC cell lines A549 and H1299 we cultured in the hEML-CM and in a tumor-conditioned medium. Morphological changes were analyzed with optical and transmission electron microscopy. To evaluate the functional effect of conditioned medium in tumor cells, we analyzed cell proliferation, migration, colony formation capacity in 2D and 3D and in vivo tumor growth capacity. The expression of the pluripotency genes OSKM, the adenocarcinoma marker NKX2-1, the lung surfactant proteins SFTP, the myofibroblast marker MYH and DNMT3A/3B was analyzed with qRT-PCR and the presence of the myofibroblast markers vimentin and α-SMA with immunofluorescence. Transcriptomic analysis was performed using Affymetrix arrays. Results: The A549 and H1299 cells cultured in hEML-CM lost their epithelial morphology, acquired mesodermal characteristics, and decreased proliferation, migration, and colony formation capacity in 2D and 3D, as well as reduced its capacity to growth in vivo. The expression of OSKM, NKX2-1 and SFTP decreased, while that of DNMT3A/3B, vimentin, α-SMA and MYH increased. Distant matrix analysis based on transcriptomic profile showed that conditioned cells were closer to myoblast and human lung fibroblast than to normal epithelial immortalized lung cells. A total of 1631 for A549 and 866 for H1299 differentially expressed genes between control and conditioned cells were identified. Conclusions: To the best of our knowledge, this is the first study to report that stimuli from the embryonic lung can modulate the malignant phenotype of lung cancer cells, control their growth capacity and activate their differentiation into myofibroblasts. These findings could lead to new strategies for lung cancer management.-
dc.format.extent16 p.-
dc.publisherBioMed Central-
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofJournal of Experimental & Clinical Cancer Research, 2022, vol. 41, num.1-
dc.rightscc-by (c) Canals, Jordi et al., 2022-
dc.sourceArticles publicats en revistes (Biomedicina)-
dc.subject.classificationCèl·lules mare embrionàries-
dc.subject.classificationTeixit connectiu-
dc.subject.classificationCèl·lules canceroses-
dc.subject.otherEmbryonic stem cells-
dc.subject.otherConnective tissue-
dc.subject.otherCancer cells-
dc.titleHuman embryonic mesenchymal lung-conditioned medium promotes differentiation to myofibroblast and loss of stemness phenotype in lung adenocarcinoma cell lines-
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Biomedicina)

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