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Title: | Leveraging single-cell genomics for cell therapy in Huntington's disease |
Author: | Lozano Gargallo, Clàudia |
Director/Tutor: | Colomer, Jordi |
Keywords: | Enginyeria biomèdica Materials biomèdics Treballs de fi de grau Biomedical engineering Biomedical materials Bachelor's theses |
Issue Date: | 5-Jun-2024 |
Abstract: | brain regions,particularlyaffectingthestriatalneuronsknownasMediumSpinyNeurons(MSNs). Unfortunately,neuronsdonotregenerate,thus,makingitimpossibleforthebodytoreplacetheir function. Despiteextensiveresearch,thereiscurrentlynocureforHD,andthegoaloftreatmentis to alleviatesymptoms. Stem celltherapyrepresentsapromisingdirection,allowingindefinite in vitro cells expansionand differentiationintothedesiredtargetneurons.Themainlimitationofneuralcellstransplantisthat once theirprojectionsaregrown,theycannotbedetractedfromthecultureplatewithoutbreaking and causingcelldeath. In thisproject,wefocusonidentifyingtheoptimaltargetprogenitorsubpopulation.Thecellsshould be matureenoughtoexclusivelydifferentiateintoMSNsbutyetnotoverlymature,preventingthe development oftheprojectionsthatleadtocelldeathuponbreaking.Leveragingcomputational genomics, weanalyzehumansingle-cellRNAsequencingdatatocharacterizetheMSNdifferen- tiation process.Integrating in vivo and in vitro data, wegroupcellsindifferentclustersdepending on theirgeneticexpressionandcomputetheirvelocitiestoanalysetheirfate.Ourfindingsreveala potential groupofcellsthatseemtofulfillallthecriteria.Additionally,theprojecthasenabledthe characterization ofthedifferentlineagesofcellswithinthedifferentiationprocessofstriatalneurons. |
Note: | Treballs Finals de Grau d'Enginyeria Biomèdica. Facultat de Medicina i Ciències de la Salut. Universitat de Barcelona. Curs: 2023-2024. Tutor/Director: Jordi Colomer ; Director: Jordi Abante |
URI: | http://hdl.handle.net/2445/213223 |
Appears in Collections: | Treballs Finals de Grau (TFG) - Enginyeria Biomèdica |
Files in This Item:
File | Description | Size | Format | |
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TFG_Lozano_Gargallo_Claudia.pdf | 8.79 MB | Adobe PDF | View/Open |
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