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http://hdl.handle.net/2445/214373
Title: | Engineering a microphysiological model for regenerative endodontic studies |
Author: | Sanz-Serrano, Diana Mercadé Bellido, Montserrat Ventura Pujol, Francesc Sánchez de Diego, Cristina |
Keywords: | Cèl·lules mare Endodòncia Microfluídica Visualització tridimensional Stem cells Endodontics Microfluidics Three-dimensional display systems |
Issue Date: | 28-Mar-2024 |
Publisher: | MDPI |
Abstract: | Dental pulp infections are common buccal diseases. When this happens, endodontic treatments are needed to disinfect and prepare the root canal for subsequent procedures. However, the lack of suitable in vitro models representing the anatomy of an immature root canal hinders research on regenerative events crucial in endodontics, such as regenerative procedures. This study aimed to develop a 3D microphysiological system (MPS) to mimic an immature root canal and assess the cytotoxicity of various irrigating solutions on stem cells. Utilizing the Dental Stem Cells SV40 (DSCS) cell line derived from human apical papilla stem cells, we analyzed the effects of different irrigants, including etidronic acid. The results indicated that irrigating solutions diminished cell viability in 2D cultures and influenced cell adhesion within the microphysiological device. Notably, in our 3D studies in the MPS, 17% EDTA and 9% 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) irrigating solutions demonstrated superior outcomes in terms of DSCS viability and adherence compared to the control. This study highlights the utility of the developed MPS for translational studies in root canal treatments and suggests comparable efficacy between 9% HEBP and 17% EDTA irrigating solutions, offering potential alternatives for clinical applications. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/biology13040221 |
It is part of: | Biology, 2024, vol. 13, num.4 |
URI: | http://hdl.handle.net/2445/214373 |
Related resource: | https://doi.org/10.3390/biology13040221 |
ISSN: | 2079-7737 |
Appears in Collections: | Articles publicats en revistes (Odontoestomatologia) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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