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Title: | Whole-rat protein content estimation: applicability of the Nx6.25 factor |
Author: | Rafecas Jorba, Immaculada Esteve Ràfols, Montserrat Fernández López, José Antonio Remesar Betlloch, Xavier Alemany, Marià, 1946- |
Keywords: | Rates (Animals de laboratori) Proteïnes dels aliments Nitrogen conversion factor (6.25) Protein analysis Protein nitrogen Rat |
Issue Date: | 1994 |
Publisher: | The Nutrition Society, 1994 |
Abstract: | The amino acid composition of the protein from three strains of rat (Wistar, Zucker lean and Zucker obese), subjected to reference and high-fat diets has been used to determine the mean empirical formula, molecular weight and N content of whole-rat protein. The combined whole protein of the rat was uniform for the six experimental groups, containing an estimate of 17.3% N and a mean aminoacyl residue molecular weight of 103.7. This suggests that the appropriate protein factor for the calculation of rat protein from its N content should be 5.77 instead of the classical 6.25. In addition, an estimate of the size of the non-protein N mass in the whole rat gave a figure in the range of 5.5 % of all N. The combination of the two calculations gives a protein factor of 5.5 for the conversion of total N into rat protein. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1079/BJN19940024 |
It is part of: | British Journal of Nutrition (1994), vol. p. 199-209 |
URI: | http://hdl.handle.net/2445/22229 |
Related resource: | http://dx.doi.org/10.1079/BJN19940024 |
ISSN: | 0966-0844 |
Appears in Collections: | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) |
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