Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/223143
Title: Investigation of mobile genetic elements and their association with antibiotic resistance genes in clinical pathogens worldwide
Author: Johansson, Markus H.K.
Petersen, Thomas N.
Nag, Sidsel
Lagermann, Timmie M.R.
Birkedahl, Laura E.K.
Tafaj, Silva
Bradbury, Susan
Collignon, Peter
Daley, Denise
Dougnon, Victorien
Fabiyi, Kafayath
Coulibaly, Boubacar
Dembélé, Réné
Magloire, Natama
Ouindgueta, Isidore J.
Hossain, Zenat Z.
Begoum, Anowara
Donchev, Deyan
Diggle, Mathew
Turnbull, LeeAnn
Lévesque, Simon
Berlinger, Livia
Søgaard, Kristine K.
Diaz Guevara, Paula
Duarte, Carolina
Maikanti, Panagiota
Amlerova, Jana
Drevinek, Pavel
Tkadlec, Jan
Dilas, Milica
Kaasch, Achim
Westh, Henrik T.
Bachtarzi, Mohamed A.
Amhis, Wahiba
Salazar, Carolina E.S.
Villacis, José E.
Domínguez Luzón, Ma. Ángeles (María Ángeles)
Berbel, Dàmaris
Duployez, Claire
Paluche, Maxime
Asante-Sefa, Solomon
Møller, Mie
Ip, Margaret
Mareković, Ivana
Pál-Sonnevend, Agnes
Cocuzza, Clementiza E.
Dambrauskiene, Asta
Macanze, Alexandre
Cossa, Anelsio
Mandomando, Inácio
Nwajiobi-Princewill, Philip
Okeke, Iruka N.
Kehinde, Aderemi O.
Adebiyi, Ini
Akintayo, Ifeoluwa
Popoola, Oluwafemi
Onipede, Anthony
Blomfeldt, Anita
Nyquist, Nora E.
Bocker, Kiri
Ussher, James
Ali, Amjad
Ullah, Nimat
Khan, Habibullah
Gustafson, Natalie W.
Jarrar, Ikhlas
Al-Hamad, Arif
Luvira, Viravarn
Paveenkittiporn, Wantana
Baran, Irmak
Mwansa, James C.L.
Sikakwa, Linda
Yamba, Kaunda
Aarestrup, Frank M.
Keywords: Antibiòtics
Bacteris patògens
Elements genètics mòbils
Antibiotics
Pathogenic bacteria
Mobile genetic elements
Issue Date: 18-Aug-2025
Publisher: Public Library of Science (PLoS)
Abstract: Objectives: Antimicrobial-resistant bacteria are a major global health threat. Mobile genetic elements (MGEs) have been crucial for spreading resistance to new bacterial species, including human pathogens. Understanding how MGEs promote resistance could be essential for prevention. Here we present an investigation of MGEs and their association with resistance genes in pathogenic bacteria collected from 59 diagnostic units during 2020, representing a snapshot of clinical infections from 35 counties worldwide. Methods: We analysed 3,095 whole-genome sequenced clinical bacterial isolates from over 100 species to study the relationship between resistance genes and MGEs. The mobiliome of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Klebsiella pneumoniae were further examined for geographic differences, as these species were prevalent in all countries. Genes potentially mobilized by MGEs were identified by finding DNA segments containing MGEs and ARGs preserved in multiple species. Network analysis was used to investigate potential MGE interactions, host range, and transmission pathways. Results: The prevalence and diversity of MGEs and resistance genes varied among species, with E. coli and S. aureus carrying more diverse elements. MGE composition differed between bacterial lineages, indicating strong vertical inheritance. 102 MGEs associated with resistance were found in multiple species, and four of these elements seemed to be highly transmissible as they were found in different phyla. We identified 21 genomic regions containing resistance genes potentially mobilized by MGEs, highlighting their importance in transmitting genes to clinically significant bacteria. Conclusion: Resistance genes are spread through various MGEs, including plasmids and transposons. Our findings suggest that multiple factors influence MGE prevalence and their transposability, thereby shaping the MGE population and transmission pathways. Some MGEs have a wider host range, which could make them more important for mobilizing genes. We also identified 103 resistance genes potentially mobilised by MGEs, which could increase their transmissibility to unrelated bacteria.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0330304
It is part of: PLoS One, 2025, vol. 20, num.8
URI: https://hdl.handle.net/2445/223143
Related resource: https://doi.org/10.1371/journal.pone.0330304
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Files in This Item:
File Description SizeFormat 
899452.pdf3.02 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons