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https://hdl.handle.net/2445/22384
Title: | Rat insulin turnover in vivo |
Author: | Cañas, Xavier Fernández López, José Antonio Ardévol Grau, Anna Adán Liébana, Cristina Esteve Ràfols, Montserrat Rafecas Jorba, Immaculada Remesar Betlloch, Xavier Alemany, Marià, 1946- |
Keywords: | Insulina Rates (Animals de laboratori) Insulin Rats as laboratory animals |
Issue Date: | 1995 |
Publisher: | The Endocrine Society |
Abstract: | Zucker lean and obese rats were injected under pentobarbital anesthesia with 125I-labeled insulin; at timed intervals from 30 to 120 sec, blood samples were extracted and used for the estimation of insulin levels by RIA. A group of rats from each series was maintained under a constant infusion of noradrenaline. For each insulin determination, a duplicate blood sample containing the same amount of insulin as that used in the RIA, but without the radioactive label, was used as a blank for insulin measurement. The radioactivity in these tubes was then used for the measurement of insulin label per ml blood. From plasma label decay curves and insulin concentrations, the insulin pool size, half-life, and rate of degradation were calculated. Obese rats had higher insulin levels (2.43 nM) and showed less effect of noradrenaline than their lean counterparts, in which insulin distribution volume shrank with noradrenaline treatment. The half-life of plasma insulin was similar in all groups (range, 226-314 sec). Pool size and overall degradation rates were higher in obese (198 femtokatals) than in lean rats (28 femtokatals). It is postulated that obese rats synthesize and cleave much more insulin than lean controls despite their higher circulating levels of insulin. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1210/en.136.9.3871 |
It is part of: | Endocrinology, 1995, vol. 136, num. 9, p. 3871-3876 |
URI: | https://hdl.handle.net/2445/22384 |
Related resource: | http://dx.doi.org/10.1210/en.136.9.3871 |
ISSN: | 0013-7227 |
Appears in Collections: | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) |
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File | Description | Size | Format | |
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110355.pdf | 4.34 MB | Adobe PDF | View/Open |
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