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Title: Rat insulin turnover in vivo
Author: Cañas, Xavier
Fernández López, José Antonio
Ardévol Grau, Anna
Adán Liébana, Cristina
Esteve Ràfols, Montserrat
Rafecas Jorba, Immaculada
Remesar Betlloch, Xavier
Alemany, Marià, 1946-
Keywords: Insulina
Rates (Animals de laboratori)
Rats as laboratory animals
Issue Date: 1995
Publisher: The Endocrine Society
Abstract: Zucker lean and obese rats were injected under pentobarbital anesthesia with 125I-labeled insulin; at timed intervals from 30 to 120 sec, blood samples were extracted and used for the estimation of insulin levels by RIA. A group of rats from each series was maintained under a constant infusion of noradrenaline. For each insulin determination, a duplicate blood sample containing the same amount of insulin as that used in the RIA, but without the radioactive label, was used as a blank for insulin measurement. The radioactivity in these tubes was then used for the measurement of insulin label per ml blood. From plasma label decay curves and insulin concentrations, the insulin pool size, half-life, and rate of degradation were calculated. Obese rats had higher insulin levels (2.43 nM) and showed less effect of noradrenaline than their lean counterparts, in which insulin distribution volume shrank with noradrenaline treatment. The half-life of plasma insulin was similar in all groups (range, 226-314 sec). Pool size and overall degradation rates were higher in obese (198 femtokatals) than in lean rats (28 femtokatals). It is postulated that obese rats synthesize and cleave much more insulin than lean controls despite their higher circulating levels of insulin.
Note: Reproducció del document publicat a:
It is part of: Endocrinology, 1995, vol. 136, num. 9, p. 3871-3876
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ISSN: 0013-7227
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

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