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Title: Plasma leptin turnover rates in lean and obese Zucker rats
Author: Vilà Pont, Ruth
Adán Liébana, Cristina
Rafecas Jorba, Immaculada
Fernández López, José Antonio
Remesar Betlloch, Xavier
Alemany, Marià, 1946-
Keywords: Metabolisme
Rates (Animals de laboratori)
Rats as laboratory animals
Issue Date: 1998
Publisher: The Endocrine Society
Abstract: Conscious female adult lean and obese Zucker rats were injected through the jugular vein with radioactive iodine-labeled murine leptin; in the ensuing 8 min, four blood samples were sequentially extracted from the carotid artery. The samples were used in a modified RIA for leptin, in which paired tubes received the same amount of either labeled or unlabeled leptin, thus allowing us to estimate both leptin levels and specific radioactivity. The data were used to determine the decay curve parameters from which the half-life of leptin (5.46 ± 0.23 min for lean rats and 6.99 ± 0.75 min for obese rats) as well as the size of its circulating pool (32 pmol/kg for lean rats and 267 pmol/kg for obese rats) and the overall degradation rate (96 fkat/kg for lean rats and 645 fkat/kg for obese rats) were estimated. These values are consistent with the hormonal role of leptin and the need for speedy changes in its levels in response to metabolic challenge.
Note: Reproducció del document publicat a:
It is part of: Endocrinology, 1998, vol. 139, p. 4466-4469
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ISSN: 0013-7227
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

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