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Title: RNA interference: a new and powerful tool for functional genomic analysis.
Author: Bueno i Torrens, David, 1965-
Romero Benedí, Rafael
Hernández Hernández, Victor
Sancho, Vanessa
Fernández-Rodríguez, Juana
Cardona, Albert T.
Vila Farré, Miquel
Keywords: Enginyeria genètica
Genetic engineering
Issue Date: 2001
Publisher: Institut d'Estudis Catalans
Abstract: In many species, the introduction of double-stranded RNA induces potent and specific gene silencing, referred to as RNA interference. This phenomenon, which is based on targeted degradation of mRNAs and occurs in almost any eukaryote, from trypanosomes to mice including plants and fungi, has sparked general interest from both applied and fundamental standpoints. RNA interference, which is currently used to investigate gene function in a variety of systems, is linked to natural resistance to viruses and transposon silencing, as if it were a primitive immune system involved in genome surveillance. Here, we review the mechanism of RNA interference in post-transcriptional gene silencing, its function in nature, its value for functional genomic analysis, and the modifications and improvements that may make it more efficient and inheritable. We also discuss the future directions of this versatile technique in both fundamental and applied science.
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It is part of: Contributions to Science, 2001, vol. 2, num. 1, p. 23-33
ISSN: 1575-6343
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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