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Title: Regulation of protein transport from the Golgi complex to the endoplasmic reticulum by CDC42 and N-WASP.
Author: Luna Moñux, Ana
Matas Guadix, Olga Belén
Martínez-Menárguez, José Ángel
Mato Matute, Maria Eugènia
Durán Serrano, Juan Manuel
Ballesta, José
Way, Michael
Egea Guri, Gustavo
Keywords: Aparell de Golgi
Reticle endoplasmàtic
Transport biològic
Golgi apparatus
Endoplasmic reticulum
Biological transport
Issue Date: 2002
Publisher: American Society for Cell Biology
Abstract: Actin is involved in the organization of the Golgi complex and Golgi-to-ER protein transport in mammalian cells. Little, however, is known about the regulation of the Golgi-associated actin cytoskeleton. We provide evidence that Cdc42, a small GTPase that regulates actin dynamics, controls Golgi-to-ER protein transport. We located GFP-Cdc42 in the lateral portions of Golgi cisternae and in COPI-coated and noncoated Golgi-associated transport intermediates. Overexpression of Cdc42 and its activated form Cdc42V12 inhibited the retrograde transport of Shiga toxin from the Golgi complex to the ER, the redistribution of the KDEL receptor, and the ER accumulation of Golgi-resident proteins induced by the active GTP-bound mutant of Sar1 (Sar1[H79G]). Coexpression of wild-type or activated Cdc42 and N-WASP also inhibited Golgito-ER transport, but this was not the case in cells expressing Cdc42V12 and N-WASP(AWA), a mutant form of N-WASP that lacks Arp2/3 binding. Furthermore, Cdc42V12 recruited GFP-NWASP to the Golgi complex. We therefore conclude that Cdc42 regulates Golgi-to-ER protein transport in an N-WASP¿dependent manner.
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It is part of: Molecular Biology of the Cell, 2002, vol. 13, núm. 3, p. 866-879
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ISSN: 1059-1524
Appears in Collections:Articles publicats en revistes (Biomedicina)

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