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Title: | Cholesterol regulates Syntaxin 6 trafficking at the TGN-endosomal boundaries |
Author: | Reverter Martín, Meritxell Rentero Alfonso, Carles García Melero, Ana Hoque, Monira Vilà de Muga, Sandra Álvarez-Guaita, Anna Conway, James Wood, Peta Cairns, Rose Lykopoulou, Lila Grinberg Vaisman, Daniel Raúl Vilageliu i Arqués, Lluïsa Bosch i Rodríguez, Marta Heeren, Joerg Blasi Cabús, Joan Timpson, Paul Pol i Sorolla, Albert Tebar Ramon, Francesc Murray, Rachael Z. Grewal, Thomas |
Keywords: | Colesterol Transport biològic Biologia molecular Aparell de Golgi Cholesterol Biological transport Molecular biology Golgi apparatus |
Issue Date: | 8-May-2014 |
Publisher: | Elsevier |
Abstract: | Inhibition of cholesterol export from late endosomes causes cellular cholesterol imbalance, including cholesterol depletion in the trans-Golgi network (TGN). Here, using Chinese hamster ovary (CHO) Niemann-Pick type C1 (NPC1) mutant cell lines and human NPC1 mutant fibroblasts, we show that altered cholesterol levels at the TGN/endosome boundaries trigger Syntaxin 6 (Stx6) accumulation into VAMP3, transferrin, and Rab11-positive recycling endosomes (REs). This increases Stx6/VAMP3 interaction and interferes with the recycling of αVβ3 and α5β1 integrins and cell migration, possibly in a Stx6-dependent manner. In NPC1 mutant cells, restoration of cholesterol levels in the TGN, but not inhibition of VAMP3, restores the steady-state localization of Stx6 in the TGN. Furthermore, elevation of RE cholesterol is associated with increased amounts of Stx6 in RE. Hence, the fine-tuning of cholesterol levels at the TGN-RE boundaries together with a subset of cholesterol-sensitive SNARE proteins may play a regulatory role in cell migration and invasion. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1016/j.celrep.2014.03.043 |
It is part of: | Cell Reports, 2014, vol. 7, num. 3, p. 883-897 |
URI: | http://hdl.handle.net/2445/54324 |
Related resource: | http://dx.doi.org/10.1016/j.celrep.2014.03.043 |
ISSN: | 2211-1247 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) Articles publicats en revistes (Biomedicina) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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