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DC Field | Value | Language |
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dc.contributor.author | Cantó, Elisabet | - |
dc.contributor.author | Garcia Planella, Esther | - |
dc.contributor.author | Zamora-Atenza, Carlos | - |
dc.contributor.author | Nieto, Juan Camilo | - |
dc.contributor.author | Gordillo, Jordi | - |
dc.contributor.author | Ortiz, Ma. Àngels | - |
dc.contributor.author | Metón Teijeiro, Isidoro | - |
dc.contributor.author | Serrano, Elena | - |
dc.contributor.author | Vegas Lozano, Esteban | - |
dc.contributor.author | García Bosch, Orlando | - |
dc.contributor.author | Juárez Rubio, Cándido | - |
dc.contributor.author | Vidal i Alcorisa, Sílvia | - |
dc.date.accessioned | 2014-07-16T09:02:16Z | - |
dc.date.available | 2014-07-16T09:02:16Z | - |
dc.date.issued | 2014-04-09 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/55853 | - |
dc.description.abstract | The exact function of interleukin-19 (IL-19) on immune response is poorly understood. In mice, IL-19 up-regulates TNFalpha and IL-6 expression and its deficiency increases susceptibility to DSS-induced colitis. In humans, IL-19 favors a Th2 response and is elevated in several diseases. We here investigate the expression and effects of IL-19 on cells from active Crohn"s disease (CD) patient. Twenty-three active CD patients and 20 healthy controls (HC) were included. mRNA and protein IL-19 levels were analyzed in monocytes. IL-19 effects were determined in vitro on the T cell phenotype and in the production of cytokines by immune cells. We observed that unstimulated and TLR-activated monocytes expressed significantly lower IL-19 mRNA in active CD patients than in HC (logFC =21.97 unstimulated; 21.88 with Pam3CSK4; and 21.91 with FSL-1; p<0.001). These results were confirmed at protein level. Exogenous IL-19 had an anti-inflammatory effect on HC but not on CD patients. IL-19 decreased TNFalpha production in PBMC (850.7675.29 pg/ml vs 2626.06350 pg/ml; p<0.01) and increased CTLA4 expression (22.0461.55% vs 13.9862.05%; p<0.05) and IL-4 production (32.568.9 pg/ml vs 13.562.9 pg/ml; p<0.05) in T cells from HC. IL-10 regulated IL-19 production in both active CD patients and HC. We observed that three of the miRNAs that can modulate IL-19 mRNA expression, were up-regulated in monocytes from active CD patients. These results suggested that IL-19 had an anti-inflammatory role in this study. Defects in IL-19 expression and the lack of response to this cytokine could contribute to inflammatory mechanisms in active CD patients. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: http://doi.org/10.1371/journal.pone.0093910 | - |
dc.relation.ispartof | PLoS One, 2014, vol. 9, num. 4, p. e93910 | - |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pone.0093910 | - |
dc.rights | cc-by (c) Cantó, Elisabet et al., 2014 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) | - |
dc.subject.classification | Cèl·lules T | - |
dc.subject.classification | Citometria de fluxe | - |
dc.subject.classification | Citoquines | - |
dc.subject.classification | Leucòcits | - |
dc.subject.classification | Malaltia de Crohn | - |
dc.subject.classification | Micro RNAs | - |
dc.subject.classification | Resposta immunitària | - |
dc.subject.classification | RNA | - |
dc.subject.other | T cells | - |
dc.subject.other | Flow cytometry | - |
dc.subject.other | Cytokines | - |
dc.subject.other | Leucocytes | - |
dc.subject.other | Crohn's disease | - |
dc.subject.other | MicroRNAs | - |
dc.subject.other | Immune response | - |
dc.subject.other | RNA | - |
dc.title | Interleukin-19 impairment in active Crohn's disease patients | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 641225 | - |
dc.date.updated | 2014-07-16T09:02:18Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 24718601 | - |
dc.identifier.pmid | 33182538 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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