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http://hdl.handle.net/2445/59423
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DC Field | Value | Language |
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dc.contributor.author | Sainz Polo, M. A. | - |
dc.contributor.author | Valenzuela Mayorga, Susana Valeria | - |
dc.contributor.author | Pastor Blasco, Francisco I. Javier | - |
dc.contributor.author | Sanz Aparicio, J. | - |
dc.date.accessioned | 2014-11-05T16:39:11Z | - |
dc.date.available | 2014-11-05T16:39:11Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.uri | http://hdl.handle.net/2445/59423 | - |
dc.description.abstract | Xyn30D, a new member of a recently identified group of xylanases, has been purified and crystallized. Xyn30D is a bimodular enzyme composed of an N-terminal catalytic domain belonging to glycoside hydrolase family 30 (GH30) and a C-terminal family 35 carbohydrate-binding domain (CBM35) able to bind xylans and glucuronic acid. Xyn30D shares the characteristic endo mode of action described for GH30 xylanases, with the hydrolysis of the [beta]-(1,4) bonds of xylan being directed by [alpha]-1,2-linked glucuronate moieties, which have to be placed at the -2 subsite of the xylanase active site. Crystals of the complete enzyme were obtained and a full data set to 2.3 Å resolution was collected using a synchrotron X-ray source. This represents the first bimodular enzyme with the domain architecture GH30-CBM35. This study will contribute to the understanding of the role that the different xylanases play in the depolymerization of glucuronoxylan. | - |
dc.format.extent | 5 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | International Union of Crystallography | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1107/S2053230X14012035 | - |
dc.relation.ispartof | Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2014, vol. F70, p. 963-966 | - |
dc.relation.uri | http://dx.doi.org/10.1107/S2053230X14012035 | - |
dc.rights | (c) International Union of Crystallography, 2014 | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Enzims microbians | - |
dc.subject.classification | Biopolímers | - |
dc.subject.other | Microbial enzymes | - |
dc.subject.other | Biopolymers | - |
dc.title | Crystallization and preliminary X-ray diffraction analysis of Xyn30D from Paenibacillus barcinonensis | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 644290 | - |
dc.date.updated | 2014-11-05T16:39:11Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 25005099 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
Files in This Item:
File | Description | Size | Format | |
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644290.pdf | 583.37 kB | Adobe PDF | View/Open |
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