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http://hdl.handle.net/2445/66705
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DC Field | Value | Language |
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dc.contributor.author | Solórzano, Carla | - |
dc.contributor.author | Srikumar, Shabarinath | - |
dc.contributor.author | Canals, Rocío | - |
dc.contributor.author | Juárez Giménez, Antonio | - |
dc.contributor.author | Paytubi Casabona, Sònia | - |
dc.contributor.author | Madrid Xufré, Cristina | - |
dc.date.accessioned | 2015-08-24T08:21:56Z | - |
dc.date.available | 2015-08-24T08:21:56Z | - |
dc.date.issued | 2015-07-30 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/2445/66705 | - |
dc.description.abstract | The Hha family of proteins is involved in the regulation of gene expression in enterobacteria by forming complexes with H-NS-like proteins. Whereas several amino acid residues of both proteins participate in the interaction, some of them play a key role. Residue D48 of Hha protein is essential for the interaction with H-NS, thus the D48N substitution in Hha protein abrogates H-NS/Hha interaction. Despite being a paralog of H-NS protein, StpA interacts with HhaD48N with higher affinity than with the wild type Hha protein. To analyze whether Hha is capable of acting independently of H-NS and StpA, we conducted transcriptomic analysis on the hha and stpA deletion strains and the hhaD48N substitution strain of Salmonella Typhimurium using a custom microarray. The results obtained allowed the identification of 120 genes regulated by Hha in an H-NS/StpA-independent manner, 38% of which are horizontally acquired genes. A significant number of the identified genes are involved in functions related to cell motility, iron uptake, and pathogenicity. Thus, motility assays, siderophore detection and intra-macrophage replication assays were performed to confirm the transcriptomic data. Our findings point out the importance of Hha protein as an independent regulator in S. Typhimurium, highlighting a regulatory role on virulence. | - |
dc.format.extent | 14 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.3389/fmicb.2015.00773 | - |
dc.relation.ispartof | Frontiers in Microbiology, 2015, vol. 6, p. 773-773 | - |
dc.relation.uri | http://dx.doi.org/10.3389/fmicb.2015.00773 | - |
dc.rights | cc-by (c) Solórzano, Carla et al., 2015 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Proteïnes | - |
dc.subject.classification | Salmonel·la | - |
dc.subject.classification | Genòmica | - |
dc.subject.other | Proteins | - |
dc.subject.other | Salmonella | - |
dc.subject.other | Genomics | - |
dc.title | Hha has a defined regulatory role that is not dependent upon H-NS or StpA | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 653906 | - |
dc.date.updated | 2015-08-24T08:21:57Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 26284052 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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653906.pdf | 1.91 MB | Adobe PDF | View/Open |
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