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Title: | Detection of Streptococcus pneumoniae and Haemophilus influenzae type B by real-time PCR from dried blood spot samples among children with pneumonia: a useful approach for developing countries |
Author: | Selva Jové, Laura Benmessaoud, Rachid Lanaspa, Miguel Jroundi, Imane Moraleda Redecilla, Cinta Acácio, Sozinho Iñigo, Melania Bastiani, Alien Monsonís, Manuel Pallarés Giner, Roman Bassat Orellana, Quique Muñoz-Almagro, Carmen |
Keywords: | Bacteris patògens Reacció en cadena de la polimerasa Pneumònia Infants Pneumococs Pathogenic bacteria Polymerase chain reaction Pneumonia Children Streptococcus pneumonia |
Issue Date: | 8-Oct-2013 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Background: Dried blood spot (DBS) is a reliable blood collection method for storing samples at room temperature and easily transporting them. We have previously validated a Real-Time PCR for detection of Streptococcus pneumoniae in DBS. The objective of this study was to apply this methodology for the diagnosis of S. pneumoniae and Haemophilus influenzae b (Hib) in DBS samples of children with pneumonia admitted to two hospitals in Mozambique and Morocco. Methods: Ply and wzg genes of S. pneumoniae and bexA gene of Hib, were used as targets of Real-Time PCR. 329 DBS samples of children hospitalized with clinical diagnosis of pneumonia were tested. Results: Real-Time PCR in DBS allowed for a significant increase in microbiological diagnosis of S. pneumoniae and Hib. When performing blood bacterial culture, only ten isolates of S. pneumoniae and none of Hib were detected (3·0% positivity rate, IC95% 1·4-5·5%). Real-Time PCR from DBS samples increased the detection yield by 4x fold, as 30 S. pneumoniae and 11 Hib cases were detected (12·4% positivity rate, IC95% 9·0-16·5%; P<0·001). Conclusion: Real-Time PCR applied in DBS may be a valuable tool for improving diagnosis and surveillance of pneumonia caused by S. pneumoniae or Hib in developing countries. |
Note: | Correction https://doi.org/10.1371/journal.pone.0147678 |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0076970 |
It is part of: | PLoS One, 2013, vol. 8, num. 10, p. e76970 |
URI: | http://hdl.handle.net/2445/69369 |
Related resource: | http://dx.doi.org/10.1371/journal.pone.0076970 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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File | Description | Size | Format | |
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640999.pdf | 187.66 kB | Adobe PDF | View/Open | |
677824_Correction.pdf | Correction | 123.07 kB | Adobe PDF | View/Open |
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