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Title: Short-term oleoyl-estrone treatment affects capacity to manage lipids in rat adipose tissue
Author: Salas Mani, Anna
Noé Mata, Verónica
Ciudad i Gómez, Carlos Julián
Romero Romero, María del Mar
Remesar Betlloch, Xavier
Esteve Ràfols, Montserrat
Keywords: Obesitat
Teixit adipós
Rates (Animals de laboratori)
Lipid Metabolism
Adipose tissues
Issue Date: 2007
Publisher: BioMed Central
Abstract: Background: Short-term OE (oleoyl-estrone) treatment causes significant decreases in rat weight mainly due to adipose tissue loss. The aim of this work was to determine if OE treatment affects the expression of genes that regulate lipid metabolism in white adipose tissue. Results: Gene expression in adipose tissue from female treated rats (48 hours) was analysed by hybridization to cDNA arrays and levels of specific mRNAs were determined by real-time PCR. Treatment with OE decreased the expression of 232 genes and up-regulated 75 other genes in mesenteric white adipose tissue. The use of real-time PCR validate that, in mesenteric white adipose tissue, mRNA levels for Lipoprotein Lipase (LPL) were decreased by 52%, those of Fatty Acid Synthase (FAS) by 95%, those of Hormone Sensible Lipase (HSL) by 32%, those of Acetyl CoA Carboxylase (ACC) by 92%, those of Carnitine Palmitoyltransferase 1b (CPT1b) by 45%, and those of Fatty Acid Transport Protein 1 (FATP1) and Adipocyte Fatty Acid Binding Protein (FABP4) by 52% and 49%, respectively. Conversely, Tumour Necrosis Factor (TNF¿) values showed overexpression (198%). Conclusion: Short-term treatment with OE affects adipose tissue capacity to extract fatty acids from lipoproteins and to deal with fatty acid transport and metabolism.
Note: Reproducció del document publicat a
It is part of: BMC Genomics, 2007, vol. 8, núm. 292
ISSN: 1471-2164
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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