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http://hdl.handle.net/2445/101324
Title: | Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes |
Author: | Calderón Domínguez, María Sebastián Muñoz, David Fucho Salvador, Raquel Weber Blattes, Minéia Mir Bonnín, Joan Francesc García-Casarrubios, Ester Obregón, Maria Jesús Zorzano Olarte, Antonio Valverde, Ángela M. Serra i Cucurull, Dolors Herrero Rodríguez, Laura |
Keywords: | Carnitina palmitoïl-transferasa 1 Teixit adipós Obesitat Carnitine palmitoyltransferase I Adipose tissues Obesity |
Issue Date: | 20-Jul-2016 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | The discovery of active brown adipose tissue (BAT) in adult humans and the fact that it is reduced in obese and diabetic patients have put a spotlight on this tissue as a key player in obesity-induced metabolic disorders. BAT regulates energy expenditure through thermogenesis; therefore, harnessing its thermogenic fat-burning power is an attractive therapeutic approach. We aimed to enhance BAT thermogenesis by increasing its fatty acid oxidation (FAO) rate. Thus, we expressed carnitine palmitoyltransferase 1AM (CPT1AM), a permanently active mutant form of CPT1A (the rate-limiting enzyme in FAO), in a rat brown adipocyte (rBA) cell line through adenoviral infection. We found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements. This indicates that CPT1AM-mediated increase in brown adipocytes FAO may be a new approach to the treatment of obesity-induced disorders. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0159399 |
It is part of: | PLoS One, 2016, vol. 11, num. 7, p. e0159399 |
URI: | http://hdl.handle.net/2445/101324 |
Related resource: | http://dx.doi.org/10.1371/journal.pone.0159399 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) Articles publicats en revistes (Institut de Biomedicina (IBUB)) Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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