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Title: | Phytohormone Profiling method for rice: effects of GA20ox mutation on the gibberellin content of japonica rice varieties |
Author: | López Cristoffanini, Camilo Alonso Serrat Gurrera, Xavier Jáuregui Pallarés, Olga Nogués Mestres, Salvador López Carbonell, Marta |
Keywords: | Arròs Biotecnologia alimentària Rice Food biotechnology |
Issue Date: | 7-Jun-2019 |
Publisher: | Frontiers Media |
Abstract: | Gibberellins (GAs) are a very important group of phytohormones involved in seed germination, vegetative growth, flowering, and fruit development, being only 4 of the 136 known bioactives: GA1, GA3, GA4 and GA7. It has been evidenced that mutations in the OsGA20ox-2 gene produce rice (Oryza sativa) dwarf varieties, which were one of the main pillars of the green revolution. In this work two main objectives were proposed: (i) develop a rapid and broad phytohormone profiling method and (ii) to study the effects on the GA content of the GA20ox-2 mutation in several rice developmental stages using three varieties (tall variety, elite variety, mutated variety). A phytohormone extraction using an SPE step and HPLC-MS/MS detection using a QqQ instrument was determined which resulted in limits of detection (LOD) and limits of quantification (LOQ) for GAs that varied between 0.1-0.7 and 0.3-2.3 pg/g of rice sample respectively, allowing highly sensitive phytohormones detection in samples. Moreover, a good reproducibility was obtained for the GAs as relative standard deviations (RSD) for a 40 ng/mL pattern varied between 0.3-0.9%. Notoriously, GA1 was absent in the coleoptile and GA4 was the GA with higher content in the majority of developmental stages. We also observed a huge large content increment increase of the 4 bioactive GAs in the internode of the flag leaf of the mutated variety that allowinged it to attain reach the same height as the elite variety. Therefore, we provide a rapid and broad phytohormonal profiling method and evidence that the GA20ox-2 mutation is not the only factor generating dwarf varieties. To our knowledge, this is the first study that it has been reported such a high number of analyzed gibberellins at the same time in rice (Oryza sativa L. ssp. japonica)Oryza sativa japonica). |
Note: | Reproducció del document publicat a: https://doi.org/10.3389/fpls.2019.00733 |
It is part of: | Frontiers in Plant Science, 2019, vol. 10, p. 733-000 |
URI: | http://hdl.handle.net/2445/140443 |
Related resource: | https://doi.org/10.3389/fpls.2019.00733 |
ISSN: | 1664-462X |
Appears in Collections: | Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals) |
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683732.pdf | 8.11 MB | Adobe PDF | View/Open |
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