Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/142787
Title: Wound healing-promoting effects stimulated by extracellular calcium and calcium-releasing nanoparticles on dermal fibroblasts
Author: Navarro Requena, Claudia
Pérez Amodio, Soledad
Castaño Linares, Óscar
Engel, Elisabeth
Keywords: Calci
Nanopartícules
Fibroblasts
Calcium
Nanoparticles
Fibroblasts
Issue Date: 28-Sep-2018
Publisher: Institute of Physics (IOP)
Abstract: Extracellular calcium has been proved to influence the healing process of injuries and could be used as a novel therapy for skin wound healing. However, a better understanding of its effect, together with a system to obtain a controlled release is needed. In this study, we examined whether the ionic dissolution of the calcium-phosphate-based ormoglass nanoparticles coded SG5 may produce a similar stimulating effect as extracellular calcium (from CaCl2) on rat dermal fibroblast in vitro. Cells were cultured in the presence of medium containing different calcium concentrations, normally ranging from 0.1 to 3.5 mM Ca2+. A concentration of 3.5 mM of CaCl2 increased metabolic activity, in vitro wound closure, matrix metalloproteinases (MMP) activity, collagen synthesis and cytokine expression, and reduced cell contraction capacity. Interestingly, the levels of migration and contraction capacity measured followed a dose-dependent behavior. In addition, media conditioned with SG5 stimulated the same activities as media conditioned with CaCl2, but undesired effects in chronic wound healing such as inflammatory factor expression and MMP activity were reduced compared to the equivalent CaCl2 concentration. In summary, calcium-releasing particles such as SG5 are potential biological-free biostimulators to be applied in dressings for chronic wound healing.
Note: Versió postprint del document publicat a: https://doi.org/10.1088/1361-6528/aad01f
It is part of: Nanotechnology, 2018, vol. 29, num. 39, p. 395102
URI: http://hdl.handle.net/2445/142787
Related resource: https://doi.org/10.1088/1361-6528/aad01f
ISSN: 0957-4484
Appears in Collections:Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB))
Articles publicats en revistes (Electrònica)

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