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http://hdl.handle.net/2445/165289
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DC Field | Value | Language |
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dc.contributor.author | Teixidó Condomines, Elisabet | - |
dc.contributor.author | Piqué Benages, Maria Esther | - |
dc.contributor.author | Gómez Catalán, Jesús | - |
dc.contributor.author | Llobet Mallafré, Joan M. (Joan Maria) | - |
dc.date.accessioned | 2020-06-12T07:18:48Z | - |
dc.date.available | 2020-06-12T07:18:48Z | - |
dc.date.issued | 2013-02-27 | - |
dc.identifier.issn | 0887-2333 | - |
dc.identifier.uri | http://hdl.handle.net/2445/165289 | - |
dc.description.abstract | In this study we analyzed some aspects of the assessment of developmental delay in the zebrafish embryotoxicity/teratogenicity test and explored the suitability of acetylcholinesterase (AChE) activity as a biochemical marker and as a higher throughput alternative to morphological endpoints such as head-trunk angle, tail length and morphological score. Embryos were exposed from 4 to 52 h post-fertilization (hpf) to a selection of known embryotoxic/teratogen compounds (valproic acid, retinoic acid, caffeine, sodium salicylate, glucose, hydroxyurea, methoxyacetic acid, boric acid and paraoxon-methyl) over a concentration range. They were evaluated for AChE activity, head-trunk angle, tail length and several qualitative parameters integrated in a morphological score. In general, the different patterns of the concentration-response curves allowed distinguishing between chemicals that produced growth retardation (valproic and methoxyacetic acid) and chemicals that produced non-growth-delay related malformations. An acceptable correlation between the morphological score, AChE activity and head-trunk angle as markers of developmental delay was observed, being AChE activity particularly sensitive to detect delay in the absence of malformations. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Ltd | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.tiv.2012.07.010 | - |
dc.relation.ispartof | Toxicology in Vitro, 2013, vol. 27, num. 1, p. 469-478 | - |
dc.relation.uri | https://doi.org/10.1016/j.tiv.2012.07.010 | - |
dc.rights | (c) Elsevier Ltd, 2013 | - |
dc.source | Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) | - |
dc.subject.classification | Enzimologia | - |
dc.subject.classification | Embriologia | - |
dc.subject.classification | Metabolisme | - |
dc.subject.classification | Acetilcolinesterasa | - |
dc.subject.classification | Nanisme | - |
dc.subject.classification | Malformacions | - |
dc.subject.classification | Peix zebra | - |
dc.subject.classification | Toxicologia | - |
dc.subject.other | Enzymology | - |
dc.subject.other | Embryology | - |
dc.subject.other | Metabolism | - |
dc.subject.other | Acetylcholinesterase | - |
dc.subject.other | Dwarfism | - |
dc.subject.other | Human abnormalities | - |
dc.subject.other | Zebra danio | - |
dc.subject.other | Toxicology | - |
dc.title | Assessment of Developmental Delay in the Zebrafish embryo Teratogenicity Assay | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 615987 | - |
dc.date.updated | 2020-06-12T07:18:48Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) |
Files in This Item:
File | Description | Size | Format | |
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615987.pdf | 880.97 kB | Adobe PDF | View/Open |
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