Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/165289
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dc.contributor.authorTeixidó Condomines, Elisabet-
dc.contributor.authorPiqué Benages, Maria Esther-
dc.contributor.authorGómez Catalán, Jesús-
dc.contributor.authorLlobet Mallafré, Joan M. (Joan Maria)-
dc.date.accessioned2020-06-12T07:18:48Z-
dc.date.available2020-06-12T07:18:48Z-
dc.date.issued2013-02-27-
dc.identifier.issn0887-2333-
dc.identifier.urihttp://hdl.handle.net/2445/165289-
dc.description.abstractIn this study we analyzed some aspects of the assessment of developmental delay in the zebrafish embryotoxicity/teratogenicity test and explored the suitability of acetylcholinesterase (AChE) activity as a biochemical marker and as a higher throughput alternative to morphological endpoints such as head-trunk angle, tail length and morphological score. Embryos were exposed from 4 to 52 h post-fertilization (hpf) to a selection of known embryotoxic/teratogen compounds (valproic acid, retinoic acid, caffeine, sodium salicylate, glucose, hydroxyurea, methoxyacetic acid, boric acid and paraoxon-methyl) over a concentration range. They were evaluated for AChE activity, head-trunk angle, tail length and several qualitative parameters integrated in a morphological score. In general, the different patterns of the concentration-response curves allowed distinguishing between chemicals that produced growth retardation (valproic and methoxyacetic acid) and chemicals that produced non-growth-delay related malformations. An acceptable correlation between the morphological score, AChE activity and head-trunk angle as markers of developmental delay was observed, being AChE activity particularly sensitive to detect delay in the absence of malformations.-
dc.format.extent10 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier Ltd-
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.tiv.2012.07.010-
dc.relation.ispartofToxicology in Vitro, 2013, vol. 27, num. 1, p. 469-478-
dc.relation.urihttps://doi.org/10.1016/j.tiv.2012.07.010-
dc.rights(c) Elsevier Ltd, 2013-
dc.subject.classificationEnzimologia-
dc.subject.classificationEmbriologia-
dc.subject.classificationMetabolisme-
dc.subject.classificationAcetilcolinesterasa-
dc.subject.classificationNanisme-
dc.subject.classificationMalformacions-
dc.subject.classificationPeix zebra-
dc.subject.classificationToxicologia-
dc.subject.otherEnzymology-
dc.subject.otherEmbryology-
dc.subject.otherMetabolism-
dc.subject.otherAcetylcholinesterase-
dc.subject.otherDwarfism-
dc.subject.otherHuman abnormalities-
dc.subject.otherZebra danio-
dc.subject.otherToxicology-
dc.titleAssessment of Developmental Delay in the Zebrafish embryo Teratogenicity Assay-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/acceptedVersion-
dc.identifier.idgrec615987-
dc.date.updated2020-06-12T07:18:48Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
Appears in Collections:Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

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