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http://hdl.handle.net/2445/165289
Title: | Assessment of Developmental Delay in the Zebrafish embryo Teratogenicity Assay |
Author: | Teixidó Condomines, Elisabet Piqué Benages, Maria Esther Gómez Catalán, Jesús Llobet Mallafré, Joan M. (Joan Maria) |
Keywords: | Enzimologia Embriologia Metabolisme Acetilcolinesterasa Nanisme Malformacions Peix zebra Toxicologia Enzymology Embryology Metabolism Acetylcholinesterase Dwarfism Human abnormalities Zebra danio Toxicology |
Issue Date: | 27-Feb-2013 |
Publisher: | Elsevier Ltd |
Abstract: | In this study we analyzed some aspects of the assessment of developmental delay in the zebrafish embryotoxicity/teratogenicity test and explored the suitability of acetylcholinesterase (AChE) activity as a biochemical marker and as a higher throughput alternative to morphological endpoints such as head-trunk angle, tail length and morphological score. Embryos were exposed from 4 to 52 h post-fertilization (hpf) to a selection of known embryotoxic/teratogen compounds (valproic acid, retinoic acid, caffeine, sodium salicylate, glucose, hydroxyurea, methoxyacetic acid, boric acid and paraoxon-methyl) over a concentration range. They were evaluated for AChE activity, head-trunk angle, tail length and several qualitative parameters integrated in a morphological score. In general, the different patterns of the concentration-response curves allowed distinguishing between chemicals that produced growth retardation (valproic and methoxyacetic acid) and chemicals that produced non-growth-delay related malformations. An acceptable correlation between the morphological score, AChE activity and head-trunk angle as markers of developmental delay was observed, being AChE activity particularly sensitive to detect delay in the absence of malformations. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.tiv.2012.07.010 |
It is part of: | Toxicology in Vitro, 2013, vol. 27, num. 1, p. 469-478 |
URI: | http://hdl.handle.net/2445/165289 |
Related resource: | https://doi.org/10.1016/j.tiv.2012.07.010 |
ISSN: | 0887-2333 |
Appears in Collections: | Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) |
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