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Title: Aeromonas bivalvium sp. nov. isolated from bivalve molluscs
Author: Miñana i Galbis, David
Farfán Sellarés, Maribel
Fusté Munné, M. Carme
Lorén Egea, José Gaspar
Keywords: Microbiologia
Genètica bacteriana
Taxonomia (Biologia)
Bacterial genetics
Taxonomy (Biology)
Issue Date: Mar-2007
Publisher: Society for General Microbiology
Abstract: A polyphasic study was performed to determine the taxonomic position of two <i>Aeromonas</i> strains, 665N and 868E<sup>T</sup>, isolated from bivalve molluscs, that could not be identified at the species level in a previous numerical taxonomy study. The DNA G+C content of these isolates was 62.3 and 62.6 mol%, respectively. Sequence analysis of the 16S rRNA gene showed that the two new strains were closely related to members of the genus <i>Aeromonas</i>. Fluorescence amplified fragment length polymorphism fingerprinting revealed that strains 665N and 868E<sup>T</sup> clustered together with a similarity of 78% but did not cluster with any of the <i>Aeromonas</i> genomospecies. DNA-DNA hybridization experiments revealed a high level of relatedness between the two new isolates (76 %) but low levels of relatedness between these and phylogenetically most closely related <i>Aeromonas</i> genomospecies (30-44 %). Useful tests for the phenotypic differentiation of strains 665N and 868E<sup>T</sup> from other mesophilic <i>Aeromonas</i> species included those for gas from glucose, lysine decarboxylase, Voges-Proskauer reaction, acid from L-arabinose, hydrolysis of aesculin and utilization of L-lactate. On the basis of genotypic and phenotypic evidence, strains 665N and 868ET are considered to represent a novel species of the genus <i>Aeromonas</i>, for which the name <i>Aeromonas bivalvium</i> sp. nov. is proposed. The type strain is 868E<sup>T</sup> (=CECT 7113<sup>T</sup>=LMG 23376<sup>T</sup>).
Note: Versió postprint del document publicat a:
It is part of: International Journal of Systematic and Evolutionary Microbiology, 2007, vol. 57, num. Pt3, p. 582-587
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ISSN: 1466-5026
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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