Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/166937
Title: Development and characterization of an allergic asthma rat model for interventional studies
Author: Périz, Marta
Pérez-Cano, Francisco J.
Rodríguez Lagunas, María José
Cambras Riu, Trinitat
Pastor Soplín, Santiago
Best, Iván
Castell, Margarida
Massot Cladera, Malen
Keywords: Asma
Temperatura corporal
Anafilaxi
Rates (Animals de laboratori)
Fisiologia patològica
Asthma
Body temperature
Anaphylaxis
Rats as laboratory animals
Pathological physiology
Issue Date: 28-May-2020
Publisher: MDPI
Abstract: Allergic asthma is one of the most common chronic diseases of the airways, however it still remains underdiagnosed and hence undertreated. Therefore, an allergic asthma rat model would be useful to be applied in future therapeutic strategy studies. The aim of the present study was to develop an objective model of allergic asthma in atopic rats that allows the induction and quantification of anaphylactic shock with quantitative variables. Female Brown Norway rats were intraperitoneally sensitized with ovalbumin (OVA), alum and Bordetella pertussis toxin and boosted a week later with OVA in alum. At day 28, all rats received an intranasal challenge with OVA. Anaphylactic response was accurately assessed by changes in motor activity and body temperature. Leukotriene concentration was determined in the bronchoalveolar lavage fluid (BALF), and total and IgE anti-OVA antibodies were quantified in blood and BALF samples. The asthmatic animals' motility and body temperature were reduced after the shock for at least 20 h. The asthmatic animals developed anti-OVA IgE antibodies both in BALF and in serum. These results show an effective and relatively rapid model of allergic asthma in female Brown Norway rats that allows the quantification of the anaphylactic response.
Note: Reproducció del document publicat a: https://doi.org/10.3390/ijms21113841
It is part of: International Journal of Molecular Sciences, 2020, vol. 21, num. 11, p. 3841
URI: http://hdl.handle.net/2445/166937
Related resource: https://doi.org/10.3390/ijms21113841
ISSN: 1661-6596
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)

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