Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/176640
Title: Whole-Genome Sequencing and Comparative Genomics of Three Helicobacter pylori Strains Isolated from the Stomach of a Patient with Adenocarcinoma
Author: Palau de Miguel, Montserrat
Piqué i Clusella, Núria
Comeau, André M.
Ramírez-Lázaro, M. José
Lario García, Sergio
Calvet, Xavier
Miñana i Galbis, David
Keywords: Helicobacter pylori
Malalties del tracte gastrointestinal
Aparell digestiu
Genètica bacteriana
Helicobacter pylori
Gastrointestinal system diseases
Digestive organs
Bacterial genetics
Issue Date: 2021
Publisher: MDPI
Abstract: Helicobacter pylori is a common pathogen associated with several severe digestive diseases. Although multiple virulence factors have been described, it is still unclear the role of virulence factors on H. pylori pathogenesis and disease progression. Whole genome sequencing could help to find genetic markers of virulence strains. In this work, we analyzed three complete genomes from isolates obtained at the same point in time from a stomach of a patient with adenocarcinoma, using multiple available bioinformatics tools. The genome analysis of the strains B508A-S1, B508A-T2A and B508A-T4 revealed that they were cagA, babA and sabB/hopO negative. The differences among the three genomes were mainly related to outer membrane proteins, methylases, restriction modification systems and flagellar biosynthesis proteins. The strain B508A-T2A was the only one presenting the genotype vacA s1, and had the most distinct genome as it exhibited fewer shared genes, higher number of unique genes, and more polymorphisms were found in this genome. With all the accumulated information, no significant differences were found among the isolates regarding virulence and origin of the isolates. Nevertheless, some B508A-T2A genome characteristics could be linked to the pathogenicity of H. pylori. Keywords: Helicobacter pylori; genomic comparison; virulence factors; gastric adenocarcinoma
Note: Reproducció del document publicat a: https://doi.org/10.3390/pathogens10030331
It is part of: Pathogens, 2021
URI: http://hdl.handle.net/2445/176640
Related resource: https://doi.org/10.3390/pathogens10030331
ISSN: 2076-0817
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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