Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/200900
Title: | 3D bioprinted functional skeletal muscle models have potential applications for studies of muscle wasting in cancer cachexia |
Author: | García Lizarribar, Andrea Villasante, Aranzazu Lopez Martin, Jose Antonio Flandez, Marta Soler Vázquez, M. Carmen Serra i Cucurull, Dolors Herrero Rodríguez, Laura Sagrera, Ana Efeyan, Alejo Samitier i Martí, Josep |
Keywords: | Enginyeria de teixits Caquèxia Materials biomèdics Malalties musculars Tissue engineering Cachexia Biomedical materials Muscular Diseases |
Issue Date: | 19-Apr-2023 |
Publisher: | Elsevier B.V. |
Abstract: | Acquired muscle diseases such as cancer cachexia are responsible for the poor prognosis of many patients suffering from cancer. In vitro models are needed to study the underlying mechanisms of those pathologies. Extrusion bioprinting is an emerging tool to emulate the aligned architecture of fibers while implementing ad- ditive manufacturing techniques in tissue engineering. However, designing bioinks that reconcile the rheological needs of bioprinting and the biological requirements of muscle tissue is a challenging matter. Here we formulate a biomaterial with dual crosslinking to modulate the physical properties of bioprinted models. We design 3D bioprinted muscle models that resemble the mechanical properties of native tissue and show improved prolif- eration and high maturation of differentiated myotubes suggesting that the GelMA-AlgMA-Fibrin biomaterial possesses myogenic properties. The electrical stimulation of the 3D model confirmed the contractile capability of the tissue and enhanced the formation of sarcomeres. Regarding the functionality of the models, they served as platforms to recapitulate skeletal muscle diseases such as muscle wasting produced by cancer cachexia. The genetic expression of 3D models demonstrated a better resemblance to the muscular biopsies of cachectic mouse models. Altogether, this biomaterial is aimed to fabricate manipulable skeletal muscle in vitro models in a non- costly, fast and feasible manner |
Note: | Reproducció del document publicat a: https://doi.org/10.1016/j.bioadv.2023.213426 |
It is part of: | Biomaterials Advances, 2023, vol. 150, p. 213426 |
URI: | http://hdl.handle.net/2445/200900 |
Related resource: | https://doi.org/10.1016/j.bioadv.2023.213426 |
ISSN: | 2772-9508 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Articles publicats en revistes (Enginyeria Electrònica i Biomèdica) Articles publicats en revistes (Institut de Biomedicina (IBUB)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
733486.pdf | 5 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License