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Title: Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana
Author: Atanasov, Kostadin Evgeniev
Barboza-Barquero, Luis
Fernández Tiburcio, Antonio
Alcázar Hernández, Rubén
Keywords: Arabidopsis thaliana
Genètica de poblacions
Arabidopsis thaliana
Population Genetics
Issue Date: 9-Mar-2016
Publisher: Frontiers Media
Abstract: Guazatine is a potent inhibitor of polyamine oxidase (PAO) activity. In agriculture, guazatine is used as non-systemic contact fungicide efficient in the protection of cereals and citrus fruits against disease. The composition of guazatine is complex, mainly constituted by a mixture of synthetic guanidated polyamines (polyaminoguanidines). Here we have studied the effects from exposure to guazatine in the weed Arabidopsis thaliana. We report that micromolar concentrations of guazatine are sufficient to inhibit growth of Arabidopsis seedlings and induce chlorosis, whereas germination is barely affected. We observed the occurrence of quantitative variation in the response to guazatine between 107 randomly chosen Arabidopsis accessions. This enabled us to undertake genome-wide association (GWA) mapping that identified a locus on chromosome one associated with guazatine tolerance. CHLOROPHYLLASE 1 (CLH1) within this locus was studied as candidate gene, together with its paralog (CLH2). The analysis of independent clh1-2, clh1-3, clh2-3, clh2-2 and double clh1-2 clh2-3 mutant alleles indicated that CLH1 and/or CLH2 loss-of-function or expression down-regulation promote guazatine tolerance in Arabidopsis. We report a natural mechanism by which Arabidopsis populations can overcome toxicity by the fungicide guazatine.
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It is part of: Frontiers in Plant Science, 2016, vol. 7, p. 401
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ISSN: 1664-462X
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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