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Title: Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell lines compared to primary osteoblasts
Author: De-Ugarte, Laura
Balcells Comas, Susana
Güerri Fernández, Robert
Grinberg Vaisman, Daniel Raúl
Diez-Perez, Adolfo
Nogués Solán, Xavier
Garcia Giralt, Natàlia
Keywords: Osteosarcoma
Issue Date: 20-Apr-2020
Publisher: MDPI
Abstract: The miR-320a regulates a number of genes involved in various physiological processes. In particular, it has been reported as a tumor suppressor in several types of human cancers and involved in osteoporotic fracture and osteoblast function. Hence, the role of miR-320a has been evaluated in tumor cells and in primary cells in a separated context, but its effect has never been explored in a comparative manner. The present study aims to evaluate the cellular effects of miR-320a on human osteosarcoma cell lines (MG-63 and U2OS) compared to that on primary human osteoblasts (hOBs). miR-320a was either overexpressed or inhibited in all cell lines, and cell proliferation and viability were analyzed. Additionally, the effects of miR-320a on matrix mineralization, alkaline phosphatase activity, and oxidative stress were also evaluated in order to assess osteoblast functionality. In osteosarcoma cells, miR-320a overexpression reduced cell viability and proliferation, while in hOB cell viability was not affected and proliferation even was increased. The overexpression of miR-320a in both osteosarcoma cells and hOBs reduced the mineralization capacity. Finally, an increased oxidative stress was detected in all cells after miR-320a overexpression mainly in osteosarcoma. In conclusion, the overexpression of miR-320a increased stress oxidation levels, which could be involved in the reduced osteoblast performance, even though the cell viability was only affected in osteosarcoma cells.
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It is part of: Applied Sciences, 2020, vol. 10, p. 2852
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ISSN: 2076-3417
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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