Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/163026
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Comas Basté, Oriol | - |
dc.contributor.author | Latorre Moratalla, Mariluz | - |
dc.contributor.author | Bernacchia, Roberta | - |
dc.contributor.author | Veciana Nogués, María Teresa | - |
dc.contributor.author | Vidal Carou, Ma. Carmen | - |
dc.date.accessioned | 2020-05-29T08:45:17Z | - |
dc.date.available | 2020-05-29T08:45:17Z | - |
dc.date.issued | 2017-10-25 | - |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.uri | http://hdl.handle.net/2445/163026 | - |
dc.description.abstract | Histamine intolerance is a disorder in the homeostasis of histamine due to a reduced intestinal degradation of this amine, mainly caused by diamine oxidase (DAO) enzyme deficiency, which provokes its accumulation in plasma and the appearance of adverse health affects. A new approach for the diagnosis of this intolerance could be the determination of histamine and its metabolites in urine. The aim of this work was to develop and validate a rapid method to determine histamine and methylhistamine in human urine by Ultra High Performance Liquid Chromatography and Fluorimetric detection (UHPLC-FL). The proposed method is a consistent procedure to determine histamine and methylhistamine in less than 11 minutes with adequate linearity and sensitivity. Relative standard deviation was always lower than 5.5%, ensuring method precision; and mean recovery was greater than 99% for both analytes. The structure of histamine and methylhistamine conjugated with OPA were confirmed by UHPLC-ITD-FTMS which enabled to unequivocally identify both analytes in standards and also in urine samples. The analysis of histamine and methylhistamine in urine samples could be a potential new approach for the routine diagnosis of histamine intolerance, more patient-friendly and with clear advantages in terms of equipment and personnel demand for sample collection in comparison with current plasmatic DAO activity determination. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.jpba.2017.06.029 | - |
dc.relation.ispartof | Journal of Pharmaceutical and Biomedical Analysis, 2017, vol. 145, p. 379-385 | - |
dc.relation.uri | https://doi.org/10.1016/j.jpba.2017.06.029 | - |
dc.rights | cc-by-nc-nd (c) Elsevier B.V., 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) | - |
dc.subject.classification | Histamina | - |
dc.subject.classification | Diamines | - |
dc.subject.classification | Tolerància | - |
dc.subject.classification | Cromatografia de líquids d'alta resolució | - |
dc.subject.classification | Orina | - |
dc.subject.classification | Humors corporals | - |
dc.subject.other | Histamine | - |
dc.subject.other | Diamines | - |
dc.subject.other | Toleration | - |
dc.subject.other | High performance liquid chromatography | - |
dc.subject.other | Urine | - |
dc.subject.other | Body fluids | - |
dc.title | New approach for the diagnosis of histamine intolerance based on the determination of histamine and methylhistamine in urine. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 674563 | - |
dc.date.updated | 2020-05-29T08:45:17Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) Articles publicats en revistes (Institut de Recerca en Nutrició i Seguretat Alimentària (INSA·UB)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
674563.pdf | 1.23 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License