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dc.contributor.authorPérez-Burgos, Rafael-
dc.contributor.authorGrzelak, Edyta-
dc.contributor.authorGokce, Gultekin-
dc.contributor.authorSaurina, Javier-
dc.contributor.authorBarbosa Torralbo, José-
dc.contributor.authorBarrón Bueno, Dolores-
dc.description.abstractThis work was focused on the comparison of two clean-up methods to be used for the simultaneous determination of seven cephalosporins in cow muscle. In particular, the performance of novel dispersive solid phase extraction (d-SPE) procedures based on QuEChERS methodologies was assessed and compared with conventional SPE. The separation and detection of the analytes using both methods was carried out by LC-MS/MS to reach enough sensitivity to be compatible with the detection of the maximum residue limits (MRL) of cephalosporins as regulated by EU directives. The optimization of the clean-up step relied on experimental design in order to find the most suitable conditions with a reduced number of assays. Besides, multi-objective responses were used to reach an overall compromise in the recovery of all analytes simultaneously. The validation of the two methods was done according to the Directive 2002/657/EC. Linearity, decision limit, detection capability, detection and quantification limits (4-50 μg kg⁻¹), precision (RSD less than 15% except for PIR) and recoveries were determined and adequate results with comparable values using QuEChERS and SPE methodologies. LOQ were better for SPE method (0.1-10 μg kg⁻¹) but both methods show LOQ below MRL values. Precision was slightly better for the QuEChERS method, that also presents better recoveries, higher than 85% except for cephalexin.-
dc.format.extent9 p.-
dc.publisherElsevier B.V.-
dc.relation.isformatofVersió postprint del document publicat a:
dc.relation.ispartofJournal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences, 2012, vol. 899, p. 57-65-
dc.rights(c) Elsevier B.V., 2012-
dc.sourceArticles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)-
dc.subject.classificationQuímica analítica-
dc.subject.classificationEspectrometria de masses-
dc.subject.classificationMedicaments antibacterians-
dc.subject.otherAnalytical chemistry-
dc.subject.otherMass spectrometry-
dc.subject.otherAntibacterial agents-
dc.titleQuEChERS methodologies as an alternative to solid phase extraction (SPE) for the determination and characterisation of residues of cephalosporins in beef muscle using LC-MS/MS-
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

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