Please use this identifier to cite or link to this item:
Title: QuEChERS methodologies as an alternative to solid phase extraction (SPE) for the determination and characterisation of residues of cephalosporins in beef muscle using LC-MS/MS
Author: Pérez-Burgos, Rafael
Grzelak, Edyta
Gokce, Gultekin
Saurina, Javier
Barbosa Torralbo, José
Barrón Bueno, Dolores
Keywords: Cefalosporines
Química analítica
Espectrometria de masses
Medicaments antibacterians
Analytical chemistry
Mass spectrometry
Antibacterial agents
Issue Date: 15-Jun-2012
Publisher: Elsevier B.V.
Abstract: This work was focused on the comparison of two clean-up methods to be used for the simultaneous determination of seven cephalosporins in cow muscle. In particular, the performance of novel dispersive solid phase extraction (d-SPE) procedures based on QuEChERS methodologies was assessed and compared with conventional SPE. The separation and detection of the analytes using both methods was carried out by LC-MS/MS to reach enough sensitivity to be compatible with the detection of the maximum residue limits (MRL) of cephalosporins as regulated by EU directives. The optimization of the clean-up step relied on experimental design in order to find the most suitable conditions with a reduced number of assays. Besides, multi-objective responses were used to reach an overall compromise in the recovery of all analytes simultaneously. The validation of the two methods was done according to the Directive 2002/657/EC. Linearity, decision limit, detection capability, detection and quantification limits (4-50 μg kg⁻¹), precision (RSD less than 15% except for PIR) and recoveries were determined and adequate results with comparable values using QuEChERS and SPE methodologies. LOQ were better for SPE method (0.1-10 μg kg⁻¹) but both methods show LOQ below MRL values. Precision was slightly better for the QuEChERS method, that also presents better recoveries, higher than 85% except for cephalexin.
Note: Versió postprint del document publicat a:
It is part of: Journal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences, 2012, vol. 899, p. 57-65
Related resource:
ISSN: 1570-0232
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

Files in This Item:
File Description SizeFormat 
609017.pdf497.93 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.