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http://hdl.handle.net/2445/171714
Title: | Differential regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica |
Author: | El Mouali Benomar, Youssef Esteva-Martínez, Guillem García-Pedemonte, David Balsalobre Parra, Carlos |
Keywords: | Salmonel·la Expressió gènica Salmonella Gene expression |
Issue Date: | 14-Oct-2020 |
Publisher: | Frontiers Media |
Abstract: | Post-transcriptional regulation mediated by regulatory small RNAs (sRNAs) has risen as a key player in fine-tuning gene expression in response to environmental stimuli. Here, we show that, in Salmonella enterica, the central metabolic regulator CRP-cAMP differentially regulates the sRNAs CsrB and CsrC in a growth phase-dependent manner. While CsrB expression remains unchanged during growth, CsrC displays a growth phase-dependent expression profile, being weakly expressed at the logarithmic growth phase and induced upon entry into stationary phase. We show that CRP-cAMP contributes to the expression pattern of CsrC by repressing its expression during the logarithmic growth phase. The CRP-cAMP mediated repression of CsrC is independent of SirA, a known transcriptional CsrB/CsrC activator. We further show that the sRNA Spot 42, which is derepressed in a Δcrp strain, upregulates CsrC during logarithmic growth. We propose a model where the growth-dependent regulation of CsrC is sustained by the CRP-cAMP-mediated repression of Spot 42. Together, our data point toward a differential regulation of the sRNAs CsrB and CsrC in response to environmental stimuli, leading to fine-tuning of gene expression via the sequestration of the RNA-binding protein CsrA. |
Note: | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2020.570536 |
It is part of: | Frontiers in Microbiology, 2020, vol. 11, p. 570536 |
URI: | http://hdl.handle.net/2445/171714 |
Related resource: | https://doi.org/10.3389/fmicb.2020.570536 |
ISSN: | 1664-302X |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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